Department of Nutrition & Metabolism, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, USA.
Muscle Nerve. 2012 Jul;46(1):51-9. doi: 10.1002/mus.23266. Epub 2012 May 29.
Resistance exercise (RE) stimulates a muscle protein anabolic response partially through enhanced satellite cell (SC) activity, however, age- and gender-related changes in SC content over a 24-h time course are not known.
Ten young (27 ± 2 years) men and women and 11 older (70 ± 2 years) men and women performed an acute bout of RE. Myofiber and SC characteristics were determined from muscle biopsies of the vastus lateralis using immunohistochemistry. Immunoblotting was used to determine phosphorylation of cyclin-dependent kinase-2 and protein expression of p27(Kip1) and cyclin D1.
Pax7+ SC were significantly increased in young men 24 h following RE. Percent SC were significantly increased in older women at 6 and 24 h following RE. Aging decreased myonuclear domain and increased protein expression of p27(Kip1) .
An acute bout of RE increases SC content in young men at 24 h and older women at 6 and 24 h.
阻力运动（RE）通过增强卫星细胞（SC）的活性部分刺激肌肉蛋白合成反应，然而，在 24 小时的时间过程中，SC 含量随年龄和性别而变化尚不清楚。
10 名年轻（27±2 岁）男性和女性以及 11 名年老（70±2 岁）男性和女性进行了一次急性 RE 运动。使用免疫组织化学技术从股外侧肌的肌肉活检中确定肌纤维和 SC 的特征。免疫印迹法用于测定细胞周期蛋白依赖性激酶-2 的磷酸化和 p27（Kip1）和细胞周期蛋白 D1 的蛋白表达。
RE 后 24 小时，年轻男性的 Pax7+ SC 显著增加。RE 后 6 和 24 小时，老年女性的百分比 SC 显著增加。衰老降低了核域，并增加了 p27（Kip1）的蛋白表达。
急性 RE 运动可增加年轻男性 24 小时和老年女性 6 小时和 24 小时的 SC 含量。
