Kozlova N V, Girshovich E S, Spirina T S
Antibiot Khimioter. 1990 Aug;35(8):41-3.
Isolation, identification and drug sensitivity assay of microorganisms from pathological materials of 177 patients with nonspecific diseases of the lungs, mainly pneumonia, were performed on blood and selective "chocolate" agars by using Baktofok-MK, a new dry nutrient basis developed by the authors. Blood and "chocolate" agars based on the Hottinger's hydrolysate were used as the control media. It was shown that with the quantitative procedure for inoculating the pathological material, the experimental media based on Baktofok-MK were much more sensitive to growth properties that the control media. That made it possible to detect larger numbers of etiologically important microbial species on the blood agar and to isolate clinical strains of Hemophilus spp. from a larger number of specimens on the "chocolate" agar.
采用作者研制的新型干燥营养基Baktofok-MK,在血液琼脂和选择性“巧克力”琼脂上,对177例以肺炎为主的非特异性肺部疾病患者病理材料中的微生物进行分离、鉴定及药敏试验。以基于霍廷格水解物的血液琼脂和“巧克力”琼脂作为对照培养基。结果表明,采用病理材料定量接种程序时,基于Baktofok-MK的实验培养基对生长特性的敏感性远高于对照培养基。这使得在血琼脂上能够检测到更多具有病因学意义的微生物种类,并在“巧克力”琼脂上从更多标本中分离出嗜血杆菌属的临床菌株。