Frolov A F, Rybalko S L, Shapiro A V, Ivanskaia N V, Vasina A G
Zh Mikrobiol Epidemiol Immunobiol. 1987 Jun(6):47-9.
The comparative study of the diagnostic value of the enzyme immunoassay (EIA), indirect immunofluorescence (IF) and countercurrent immunoelectrophoresis (CIE) was made. The serological identification of the isolated and reference pneumococci (19) and H. influenzae (38) strains revealed the possibility of using all three microanalytical methods for this purpose. The study of pneumococcal and H. influenzae antigens in native sputum obtained from 74 patients with acute pneumonia showed that EIA and indirect IF were highly sensitive, their sensitivity considerably exceeding that of the bacteriological analysis. Pneumococcal antigens were detected in 66.2% of patients by EIA and in 54.0% of patients by indirect IF, while H. influenzae antigens were detected in 58.1% of patients by EIA and in 67.6% of patients by indirect IF. The sensitivity of CIE proved to be considerably lower; in the detection of pneumococcal antigens it was level with the sensitivity of the bacteriological analysis (23.0%) and H. influenzae antigens could be detected only in 27.0% of patients.
对酶免疫测定（EIA）、间接免疫荧光法（IF）和对流免疫电泳（CIE）的诊断价值进行了比较研究。对分离出的肺炎球菌（19株）和流感嗜血杆菌（38株）菌株以及参考菌株进行血清学鉴定，结果表明所有这三种微量分析方法都可用于此目的。对74例急性肺炎患者的天然痰液中的肺炎球菌和流感嗜血杆菌抗原进行研究发现，EIA和间接免疫荧光法高度敏感，其敏感性大大超过细菌学分析。通过EIA在66.2%的患者中检测到肺炎球菌抗原，通过间接免疫荧光法在54.0%的患者中检测到；通过EIA在58.1%的患者中检测到流感嗜血杆菌抗原，通过间接免疫荧光法在67.6%的患者中检测到。事实证明，CIE的敏感性要低得多；在检测肺炎球菌抗原方面，其敏感性与细菌学分析相当（23.0%），而流感嗜血杆菌抗原仅在27.0%的患者中检测到。
