Petiushenko R M, Ukhabotina L S, Krakhmaleva I N, Telesnina G N, Danilenko V N, Sazykin Iu O, Navashin S M
Antibiot Khimioter. 1990 Aug;35(8):7-9.
A bireplicone plasmid pSU901,4.6 kb in length, was constructed on the basis of plasmid pUC19 and the pstIB fragment, 1.9 kb in length, from mitochondrial DNA of A. chrysogenum. Based on the hybrid plasmid pSU901 and kanamycin resistance determinant, an autonomically replicating vector for A. chrysogenum, a culture producing cephalosporin C, is being constructed.
构建了一个长度为4.6 kb的双复制子质粒pSU901,它基于质粒pUC19和来自产黄青霉线粒体DNA的长度为1.9 kb的pstIB片段。基于杂交质粒pSU901和卡那霉素抗性决定簇,正在构建一种用于产黄青霉(一种生产头孢菌素C的培养物)的自主复制载体。