Petiushenko R M, Telesnina G N, Krakhmaleva I N, Zhukov V G, Afonin V I, Sazykin Iu O, Navashin S M
Antibiot Khimioter. 1990 Apr;35(4):3-6.
Circular mDNAs 26.85 and 26.94 kb in length were isolated from two isogenic strains of A. chrysogenum producing cephalosporin C. The strains differed in antibiotic production capacity. Restriction analysis of the mDNAs was performed with using 6 endonucleases. Comparison of the restriction data revealed identity of mDNAs. A restriction map of the mDNAs was constructed. It is useful as a basis for further studies with molecular cloning.
从两株产头孢菌素C的产黄青霉同基因菌株中分离出长度分别为26.85 kb和26.94 kb的环状线粒体DNA。这两株菌株在抗生素生产能力上有所不同。使用6种核酸内切酶对线粒体DNA进行了限制性分析。对限制性分析数据的比较揭示了线粒体DNA的一致性。构建了线粒体DNA的限制性图谱。它可作为进一步进行分子克隆研究的基础。
