UMR BIOGECO INRA 1202, Ecology of Communities, University of Bordeaux 1, Bât B2 RdC Est, Avenue des Facultés, 33405 Talence, France.
Environ Monit Assess. 2013 Feb;185(2):2039-53. doi: 10.1007/s10661-012-2686-x. Epub 2012 Jun 1.
Is identification of seed bank (SB) species useful for sustainable management of vegetation restoration on Cu-contaminated soils? How does Cu contamination of the soil affect the SB and can incorporating compost into Cu-contaminated soils counter the effects of Cu? The topsoil SB was investigated at seven contaminated sub-sites of a wood preservation site. The germination parameters of the seeds were recorded using three substrates: a washed river sand (Sand), the same sand spiked with CuSO(4) to reach the same Cu concentrations as in the soil pore water (0.3 to 3.2 mg Cu/L) (Cu), and the same Cu-spiked sand amended with compost (CPM). The total number of germinated seeds (NGS) was 1,081. The whole seedling dataset enabled 12 plant species and eight families to be identified in the SB. Species richness and Shannon indexes were low. The addition of Cu in the germination substrate enhanced total NGS at one sub-site and the addition of CPM increased plant diversity at three sub-sites. SB composition varied with the sub-site but did not correlate with total soil Cu or with the Cu concentration in the soil pore water. Three species belonging to the Poaceae family dominated. In terms of total NGS, the dominant species were Portulaca oleracea and Agrostis capillaris. Similarities between SB and established vegetation were low but increased when the soil bulk density was reduced. The Cu-tolerant species P. oleracea and A. capillaris dominated in both the SB and the established vegetation. However, the pattern of SB and established vegetation differed and consequently SB was not a sufficient indicator to predict the future vegetation.
种子库（SB）物种的鉴定是否有助于受铜污染土壤的植被恢复的可持续管理？土壤铜污染如何影响 SB，并且将堆肥掺入受铜污染的土壤中能否抵消铜的影响？在木材防腐剂场地的七个受污染分站点调查了表土 SB。使用三种基质记录种子的发芽参数：水洗河沙（Sand）、与 CuSO4 混合以达到与土壤孔隙水中相同的 Cu 浓度（0.3 至 3.2mg Cu/L）的相同沙（Cu），以及添加堆肥的相同 Cu 混合沙（CPM）。发芽种子的总数（NGS）为 1081。整个幼苗数据集使 SB 中鉴定出 12 种植物和 8 科。物种丰富度和 Shannon 指数较低。在发芽基质中添加 Cu 会增加一个分站点的总 NGS，而添加 CPM 会增加三个分站点的植物多样性。SB 组成随分站点而变化，但与总土壤 Cu 或土壤孔隙水中的 Cu 浓度无关。属于禾本科的三个物种占主导地位。就总 NGS 而言，优势种为马齿苋和狗尾草。SB 与已建立植被之间的相似性较低，但当土壤容重降低时，相似性增加。耐铜物种马齿苋和狗尾草在 SB 和已建立植被中均占主导地位。然而，SB 和已建立植被的模式不同，因此 SB 不是预测未来植被的充分指标。
