Lescure A M, Massenet O, Briat J F
Laboratoire de Biologie Moléculaire Végétale, Centre National de la Recherche Scientifique (Unité de Recherche Associée n degrees 1178), Grenoble, France.
Biochem J. 1990 Nov 15;272(1):147-50. doi: 10.1042/bj2720147.
Ferric citrate induces ferritin synthesis and accumulation in soybean (Glycine max) cell suspension cultures [Proudhon, Briat & Lescure (1989) Plant Physiol. 90, 586-590]. This iron-induced ferritin has been purified from cells grown for 72 h in the presence of either 100 microM- or 500 microM-ferric citrate. It has a molecular mass of about 600 kDa and is built up from a 28 kDa subunit which is recognized by antibodies raised against pea (Pisum sativum) seed ferritin and it has the same N-terminal sequence as this latter, except for residue number 3, which is alanine in pea seed ferritin instead of valine in iron-induced soybean cell ferritin. It contains an average of 1800 atoms of iron per molecule whatever the ferric citrate concentration used to induce its synthesis. It is shown that the presence of 100 microM- or 500 microM-ferric citrate in the culture medium leads respectively to an 11- and 28-fold increase in the total intracellular iron concentration and to a 30- and 60-fold increase in the ferritin concentration. However, the percentage of iron stored in the mineral core of ferritin remains constant whatever the ferric citrate concentration used and represents only 5-6% of cellular iron.
柠檬酸铁可诱导大豆（Glycine max）细胞悬浮培养物中铁蛋白的合成与积累[普劳东、布里亚和莱斯屈尔（1989年），《植物生理学》90卷，第586 - 590页]。这种铁诱导的铁蛋白已从在100微摩尔或500微摩尔柠檬酸铁存在下培养72小时的细胞中纯化出来。它的分子量约为600 kDa，由一个28 kDa的亚基组成，该亚基可被针对豌豆（Pisum sativum）种子铁蛋白产生的抗体识别，并且除了第3位残基外，它与后者具有相同的N端序列，豌豆种子铁蛋白中的第3位残基是丙氨酸，而铁诱导的大豆细胞铁蛋白中的是缬氨酸。无论用于诱导其合成的柠檬酸铁浓度如何，每个分子平均含有1800个铁原子。结果表明，培养基中100微摩尔或500微摩尔柠檬酸铁的存在分别导致细胞内总铁浓度增加11倍和28倍，铁蛋白浓度增加30倍和60倍。然而，无论使用何种柠檬酸铁浓度，储存在铁蛋白矿质核心中的铁的百分比保持恒定，仅占细胞铁的5 - 6%。
