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1
Coupling of muscarinic receptors to the mobilization of intracellular Ca2+ stores in permeabilized SH-SY5Y human neuroblastoma cells.毒蕈碱受体与通透的人神经母细胞瘤SH-SY5Y细胞内钙库动员的偶联。
Biochem J. 1990 Nov 15;272(1):269-72. doi: 10.1042/bj2720269.
2
A comparison between muscarinic receptor occupancy, inositol 1,4,5-trisphosphate accumulation and Ca2+ mobilization in permeabilized SH-SY5Y neuroblastoma cells.在通透的SH-SY5Y神经母细胞瘤细胞中，毒蕈碱受体占有率、肌醇1,4,5-三磷酸积累与Ca2+动员之间的比较。
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3
Chronic muscarinic stimulation of SH-SY5Y neuroblastoma cells suppresses inositol 1,4,5-trisphosphate action. Parallel inhibition of inositol 1,4,5-trisphosphate-induced Ca2+ mobilization and inositol 1,4,5-trisphosphate binding.慢性毒蕈碱刺激SH-SY5Y神经母细胞瘤细胞可抑制1,4,5-三磷酸肌醇的作用。同时抑制1,4,5-三磷酸肌醇诱导的Ca2+动员和1,4,5-三磷酸肌醇结合。
J Biol Chem. 1991 Nov 25;266(33):22234-41.
4
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5
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Br J Pharmacol. 1997 Feb;120(3):455-63. doi: 10.1038/sj.bjp.0700920.
6
Inositol-1,3,4,5-tetrakisphosphate induces calcium mobilization via the inositol-1,4,5-trisphosphate receptor in SH-SY5Y neuroblastoma cells.肌醇-1,3,4,5-四磷酸通过肌醇-1,4,5-三磷酸受体在SH-SY5Y神经母细胞瘤细胞中诱导钙动员。
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7
Phosphoinositide hydrolysis in permeabilized SH-SY5Y human neuroblastoma cells is inhibited by mastoparan.在通透化的SH-SY5Y人神经母细胞瘤细胞中，磷肌醇水解受到mastoparan的抑制。
FEBS Lett. 1989 Apr 24;247(2):341-4. doi: 10.1016/0014-5793(89)81366-3.
8
Reduction of muscarinic receptor density and of guanine nucleotide-stimulated phosphoinositide hydrolysis in human SH-SY5Y neuroblastoma cells following long-term treatment with 12-O-tetradecanoylphorbol 13-acetate or mezerein.用12 - O - 十四烷酰佛波醇13 - 乙酸酯或狼毒素长期处理人SH - SY5Y神经母细胞瘤细胞后，毒蕈碱受体密度及鸟嘌呤核苷酸刺激的磷酸肌醇水解的降低。
J Neurochem. 1990 May;54(5):1725-34. doi: 10.1111/j.1471-4159.1990.tb01227.x.
9
Inhibition of inositol 1,4,5-trisphosphate metabolism in permeabilised SH-SY5Y human neuroblastoma cells by a phosphorothioate-containing analogue of inositol 1,4,5-trisphosphate.含硫代磷酸酯的肌醇1,4,5-三磷酸类似物对通透化的SH-SY5Y人神经母细胞瘤细胞中肌醇1,4,5-三磷酸代谢的抑制作用。
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10
Regulation of muscarinic agonist-induced activation of phosphoinositidase C in electrically permeabilized SH-SY5Y human neuroblastoma cells by guanine nucleotides.鸟嘌呤核苷酸对毒蕈碱激动剂诱导的电通透化SH-SY5Y人神经母细胞瘤细胞中磷酸肌醇酶C激活的调节作用。
J Neurochem. 1990 Feb;54(2):676-85. doi: 10.1111/j.1471-4159.1990.tb01924.x.

引用本文的文献

1
Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.毒蕈碱受体刺激的SH-SY5Y神经母细胞瘤细胞中肌醇三磷酸（Ins(1,4,5)P3）和肌醇四磷酸（Ins(1,3,4,5)P4）的积累与代谢
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj2730791.
2
Differences in the functional responses of two cell lines each expressing Pi-hydrolysis-coupled muscarinic receptors.
Neurochem Res. 1992 Apr;17(4):375-9. doi: 10.1007/BF00974580.
3
Elevation of cytosolic calcium by cholinoceptor agonists in SH-SY5Y human neuroblastoma cells: estimation of the contribution of voltage-dependent currents.胆碱能受体激动剂使SH-SY5Y人神经母细胞瘤细胞胞质钙升高：电压依赖性电流贡献的评估
Br J Pharmacol. 1992 Sep;107(1):207-14. doi: 10.1111/j.1476-5381.1992.tb14488.x.

本文引用的文献

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myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.肌醇1,4,5-三磷酸。肝脏中激素动员细胞内钙离子的第二信使。
J Biol Chem. 1984 Mar 10;259(5):3077-81.
2
Regulation of cytosolic free Ca2+ concentration in acinar cells of rat pancreas.大鼠胰腺腺泡细胞胞质游离钙离子浓度的调节
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3
Relationship between inositol polyphosphate production and the increase of cytosolic free Ca2+ induced by vasopressin in isolated hepatocytes.离体肝细胞中肌醇多磷酸生成与血管加压素诱导的胞质游离钙离子增加之间的关系。
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Stoichiometry of contraction and Ca2+ mobilization by inositol 1,4,5-trisphosphate in isolated gastric smooth muscle cells.
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The intact human neuroblastoma cell (SH-SY5Y) exhibits high-affinity [3H]pirenzepine binding associated with hydrolysis of phosphatidylinositols.完整的人神经母细胞瘤细胞（SH-SY5Y）表现出与磷脂酰肌醇水解相关的高亲和力[3H]哌仑西平结合。
J Neurochem. 1988 May;50(5):1513-21. doi: 10.1111/j.1471-4159.1988.tb03038.x.
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Inositol trisphosphate and diacylglycerol: two interacting second messengers.肌醇三磷酸和二酰甘油：两种相互作用的第二信使。
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G proteins: transducers of receptor-generated signals.G蛋白：受体产生信号的转导分子。
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Intracellular calcium translocation: mechanism of activation by guanine nucleotides and inositol phosphates.
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9
Depolarization of human neuroblastoma cells as a result of muscarinic receptor-induced rise in cytosolic Ca2+.毒蕈碱受体诱导的胞质Ca2+升高导致人神经母细胞瘤细胞去极化。
FEBS Lett. 1989 Jan 2;242(2):337-40. doi: 10.1016/0014-5793(89)80497-1.
10
Mass measurements of inositol(1,4,5)trisphosphate in rat cerebral cortex slices using a radioreceptor assay: effects of neurotransmitters and depolarization.采用放射受体分析法对大鼠大脑皮层切片中肌醇（1,4,5）三磷酸进行定量测定：神经递质和去极化的影响
Biochem Biophys Res Commun. 1988 Dec 15;157(2):684-91. doi: 10.1016/s0006-291x(88)80304-8.

毒蕈碱受体与通透的人神经母细胞瘤SH-SY5Y细胞内钙库动员的偶联。

Coupling of muscarinic receptors to the mobilization of intracellular Ca2+ stores in permeabilized SH-SY5Y human neuroblastoma cells.

作者信息

Wojcikiewicz R J, Safrany S T, Challiss R A, Strupish J, Nahorski S R

机构信息

Department of Pharmacology and Therapeutics, University of Leicester, U.K.

出版信息

Biochem J. 1990 Nov 15;272(1):269-72. doi: 10.1042/bj2720269.

DOI:10.1042/bj2720269
PMID:2264832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149690/
Abstract

Intracellular stores of Ca2+ were mobilized transiently by carbachol in suspensions of electrically permeabilized SH-SY5Y cells. The kinetics and the dose-dependence of this mobilization paralleled carbachol-induced increases in inositol 1,4,5-trisphosphate (InsP3) mass [for both parameters EC50 (concn. giving half-maximal response) = 60-70 microM]. Guanosine 5'-[gamma-thio]triphosphate enhanced the maximal effect and the potency of carbachol on Ca2+ mobilization and InsP3 mass, but caused separation of the dose-response curves (EC50 = 0.6 microM and 5.6 microM respectively). These data show that functional coupling of muscarinic receptors to Ca2+ mobilization can be maintained after permeabilization, reveal major effects of guanine nucleotides on agonist-induced Ca2+ mobilization and provide a basis for explanation of discrepancies between agonist potency on InsP3 concentration and Ca2+ mobilization in intact cells.

摘要

在电通透的SH-SY5Y细胞悬液中，卡巴胆碱可短暂动员细胞内的Ca2+储存。这种动员的动力学和剂量依赖性与卡巴胆碱诱导的肌醇1,4,5-三磷酸（InsP3）量的增加相似[两个参数的半数有效浓度（产生最大反应一半时的浓度）EC50 = 60 - 70微摩尔]。鸟苷5'-[γ-硫代]三磷酸增强了卡巴胆碱对Ca2+动员和InsP3量的最大效应及效力，但导致剂量反应曲线分离（EC50分别为0.6微摩尔和5.6微摩尔）。这些数据表明，毒蕈碱受体与Ca2+动员的功能偶联在通透后仍可维持，揭示了鸟嘌呤核苷酸对激动剂诱导的Ca2+动员的主要影响，并为解释完整细胞中激动剂对InsP3浓度和Ca2+动员的效力差异提供了基础。