毒蕈碱受体刺激的SH-SY5Y神经母细胞瘤细胞中肌醇三磷酸（Ins(1,4,5)P3）和肌醇四磷酸（Ins(1,3,4,5)P4）的积累与代谢
Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.
作者信息
Lambert D G, Challiss R A, Nahorski S R
机构信息
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
出版信息
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj2730791.
Abstract
Stimulation of M3 muscarinic receptors expressed by SH-SY5Y cells induced a dose- and time-related increase in the mass of Ins(1,4,5)P3 (basal 38.3 +/- 5.8 pmol/mg of protein) and Ins(1,3,4,5)P4 (basal 6.1 +/- 1.2 pmol/mg of protein). Comparison of radioreceptor mass assays with [3H]inositol labelling showed higher-fold stimulations with the former protocol. The later accumulation of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass was dependent upon extracellular Ca2+.
摘要
由SH-SY5Y细胞表达的M3毒蕈碱受体的刺激导致Ins(1,4,5)P3（基础值为38.3±5.8 pmol/mg蛋白质）和Ins(1,3,4,5)P4（基础值为6.1±1.2 pmol/mg蛋白质）的量呈剂量和时间依赖性增加。放射性受体质量测定与[3H]肌醇标记的比较显示，前一种方案的刺激倍数更高。Ins(1,4,5)P3和Ins(1,3,4,5)P4量的后期积累依赖于细胞外Ca2+。
相似文献
1
Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.毒蕈碱受体刺激的SH-SY5Y神经母细胞瘤细胞中肌醇三磷酸（Ins(1,4,5)P3）和肌醇四磷酸（Ins(1,3,4,5)P4）的积累与代谢
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj2730791.
2
Quantitative comparisons of muscarinic and bradykinin receptor-mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells.人神经母细胞瘤细胞中M胆碱能和缓激肽受体介导的肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）积累及Ca2+信号传导的定量比较
Br J Pharmacol. 1995 Mar;114(6):1133-42. doi: 10.1111/j.1476-5381.1995.tb13325.x.
3
4
Inhibition of inositol 1,4,5-trisphosphate metabolism in permeabilised SH-SY5Y human neuroblastoma cells by a phosphorothioate-containing analogue of inositol 1,4,5-trisphosphate.含硫代磷酸酯的肌醇1,4,5-三磷酸类似物对通透化的SH-SY5Y人神经母细胞瘤细胞中肌醇1,4,5-三磷酸代谢的抑制作用。
Eur J Biochem. 1990 Sep 11;192(2):459-67. doi: 10.1111/j.1432-1033.1990.tb19248.x.
5
Determination of mass changes in phosphatidylinositol 4,5-bisphosphate and evidence for agonist-stimulated metabolism of inositol 1,4,5-trisphosphate in airway smooth muscle.气道平滑肌中磷脂酰肌醇4,5-二磷酸质量变化的测定及激动剂刺激的肌醇1,4,5-三磷酸代谢的证据
Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):373-9. doi: 10.1042/bj2750373.
6
Time-dependent effects of lithium on the agonist-stimulated accumulation of second messenger inositol 1,4,5-trisphosphate in SH-SY5Y human neuroblastoma cells.锂对SH-SY5Y人神经母细胞瘤细胞中激动剂刺激的第二信使肌醇1,4,5-三磷酸积累的时间依赖性影响。
Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj3110225.
7
Receptor-mediated inositol phosphate formation in relation to calcium mobilization: a comparison of two cell lines.受体介导的肌醇磷酸形成与钙动员的关系：两种细胞系的比较
Mol Pharmacol. 1987 Sep;32(3):376-83.
8
Depolarization and agonist-stimulated changes in inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate mass accumulation in rat cerebral cortex.
J Neurochem. 1991 Sep;57(3):1042-51. doi: 10.1111/j.1471-4159.1991.tb08255.x.
9
Muscarinic-receptor-mediated changes in intracellular Ca2+ and inositol 1,4,5-trisphosphate mass in a human neuroblastoma cell line, SH-SY5Y.毒蕈碱受体介导的人神经母细胞瘤细胞系SH-SY5Y细胞内钙离子及肌醇1,4,5-三磷酸含量的变化
Biochem J. 1990 Jan 15;265(2):555-62. doi: 10.1042/bj2650555.
10
[3H]inositol polyphosphate metabolism in muscarinic cholinoceptor-stimulated airways smooth muscle: accumulation of [3H]inositol 4,5 bisphosphate via a lithium-sensitive inositol polyphosphate 1-phosphatase.毒蕈碱型胆碱能受体刺激的气道平滑肌中[3H]肌醇多磷酸代谢：通过锂敏感的肌醇多磷酸1-磷酸酶积累[3H]肌醇4,5-二磷酸
J Pharmacol Exp Ther. 1997 Feb;280(2):974-82.
引用本文的文献
1
Activation of phospholipase C in SH-SY5Y neuroblastoma cells by potassium-induced calcium entry.钾离子诱导的钙离子内流激活SH-SY5Y神经母细胞瘤细胞中的磷脂酶C
Br J Pharmacol. 1995 Sep;116(2):1797-800. doi: 10.1111/j.1476-5381.1995.tb16665.x.
2
Characterization of the effects of lithium on phosphatidylinositol (PI) cycle activity in human muscarinic m1 receptor-transfected CHO cells.锂对人毒蕈碱m1受体转染的CHO细胞中磷脂酰肌醇（PI）循环活性影响的表征
Br J Pharmacol. 1993 Oct;110(2):809-15. doi: 10.1111/j.1476-5381.1993.tb13884.x.
3
Studies on the mechanism of [3H]-noradrenaline release from SH-SY5Y cells: the role of Ca2+ and cyclic AMP.SH-SY5Y细胞中[3H]-去甲肾上腺素释放机制的研究：钙离子和环磷酸腺苷的作用
Br J Pharmacol. 1994 Mar;111(3):787-92. doi: 10.1111/j.1476-5381.1994.tb14806.x.
4
Tissue- and cell-specific expression of Ins(1,4,5)P3 3-kinase isoenzymes.
Biochem J. 1995 Mar 1;306 ( Pt 2)(Pt 2):429-35. doi: 10.1042/bj3060429.
5
Stimulation of tyrosine phosphorylation and mitogen-activated-protein (MAP) kinase activity in human SH-SY5Y neuroblastoma cells by carbachol.卡巴胆碱对人SH-SY5Y神经母细胞瘤细胞中酪氨酸磷酸化和丝裂原活化蛋白（MAP）激酶活性的刺激作用。
Biochem J. 1993 Sep 1;294 ( Pt 2)(Pt 2):545-50. doi: 10.1042/bj2940545.
6
Quantitative comparisons of muscarinic and bradykinin receptor-mediated Ins (1,4,5)P3 accumulation and Ca2+ signalling in human neuroblastoma cells.人神经母细胞瘤细胞中M胆碱能和缓激肽受体介导的肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）积累及Ca2+信号传导的定量比较
Br J Pharmacol. 1995 Mar;114(6):1133-42. doi: 10.1111/j.1476-5381.1995.tb13325.x.
7
Time-dependent effects of lithium on the agonist-stimulated accumulation of second messenger inositol 1,4,5-trisphosphate in SH-SY5Y human neuroblastoma cells.锂对SH-SY5Y人神经母细胞瘤细胞中激动剂刺激的第二信使肌醇1,4,5-三磷酸积累的时间依赖性影响。
Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj3110225.
8
Interactions between inositol tris- and tetrakis-phosphates. Effects on intracellular Ca2+ mobilization in SH-SY5Y cells.肌醇三磷酸和四磷酸之间的相互作用。对SH-SY5Y细胞内钙离子动员的影响。
Biochem J. 1991 May 15;276 ( Pt 1)(Pt 1):163-7. doi: 10.1042/bj2760163.
9
Different pathways of inositol phosphate metabolism in intact neonatal rat hearts and isolated cardiomyocytes.新生大鼠完整心脏和分离心肌细胞中肌醇磷酸代谢的不同途径。
Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):683-8. doi: 10.1042/bj2810683.
10
Elevation of cytosolic calcium by cholinoceptor agonists in SH-SY5Y human neuroblastoma cells: estimation of the contribution of voltage-dependent currents.胆碱能受体激动剂使SH-SY5Y人神经母细胞瘤细胞胞质钙升高：电压依赖性电流贡献的评估
Br J Pharmacol. 1992 Sep;107(1):207-14. doi: 10.1111/j.1476-5381.1992.tb14488.x.
本文引用的文献
1
Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.膜磷酸肌醇在激动剂依赖性水解后肌醇磷酸水平的变化。
Biochem J. 1983 May 15;212(2):473-82. doi: 10.1042/bj2120473.
2
Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.毒蕈碱受体刺激大鼠大脑皮层切片后肌醇1,3,4,5-四磷酸的快速形成。
Biochem J. 1985 Nov 15;232(1):211-5. doi: 10.1042/bj2320211.
3
Generation of inositol phosphates, cytosolic Ca2+, and ionic fluxes in PC12 cells treated with bradykinin.缓激肽处理的PC12细胞中肌醇磷酸、胞质Ca2+和离子通量的产生
J Biol Chem. 1988 Nov 25;263(33):17350-9.
4
Accumulation of inositol polyphosphate isomers in agonist-stimulated cerebral-cortex slices. Comparison with metabolic profiles in cell-free preparations.激动剂刺激的大脑皮质切片中肌醇多磷酸异构体的积累。与无细胞制剂中代谢谱的比较。
Biochem J. 1989 Feb 15;258(1):23-32. doi: 10.1042/bj2580023.
5
Mass measurements of inositol(1,4,5)trisphosphate in rat cerebral cortex slices using a radioreceptor assay: effects of neurotransmitters and depolarization.采用放射受体分析法对大鼠大脑皮层切片中肌醇（1,4,5）三磷酸进行定量测定：神经递质和去极化的影响
Biochem Biophys Res Commun. 1988 Dec 15;157(2):684-91. doi: 10.1016/s0006-291x(88)80304-8.
6
A putative M3 muscarinic cholinergic receptor of high molecular weight couples to phosphoinositide hydrolysis in human SK-N-SH neuroblastoma cells.一种假定的高分子量M3毒蕈碱胆碱能受体与人类SK-N-SH神经母细胞瘤细胞中的磷酸肌醇水解偶联。
J Neurochem. 1988 Mar;50(3):984-7. doi: 10.1111/j.1471-4159.1988.tb03008.x.
7
Muscarinic receptor binding characteristics of a human neuroblastoma SK-N-SH and its clones SH-SY5Y and SH-EP1.人神经母细胞瘤SK-N-SH及其克隆株SH-SY5Y和SH-EP1的毒蕈碱受体结合特性
Eur J Pharmacol. 1989 Jun 8;165(1):71-7. doi: 10.1016/0014-2999(89)90771-1.
8
Muscarinic receptors coupled to phosphoinositide hydrolysis and elevated cytosolic calcium in a human neuroblastoma cell line SK-N-SH.毒蕈碱受体与人类神经母细胞瘤细胞系SK-N-SH中的磷酸肌醇水解和胞质钙升高相关。
Br J Pharmacol. 1989 Dec;98(4):1328-34. doi: 10.1111/j.1476-5381.1989.tb12681.x.
9
Mass changes in inositol tetrakis- and pentakisphosphate isomers induced by chemotactic peptide stimulation in HL-60 cells.
J Biol Chem. 1989 Nov 5;264(31):18489-93.
10
Inositol phosphates and cell signalling.肌醇磷酸酯与细胞信号传导
Nature. 1989 Sep 21;341(6239):197-205. doi: 10.1038/341197a0.
Zotero 插件