毒蕈碱受体刺激的SH-SY5Y神经母细胞瘤细胞中肌醇三磷酸（Ins(1,4,5)P3）和肌醇四磷酸（Ins(1,3,4,5)P4）的积累与代谢
Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.
作者信息
Lambert D G, Challiss R A, Nahorski S R
机构信息
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
出版信息
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj2730791.
Abstract
Stimulation of M3 muscarinic receptors expressed by SH-SY5Y cells induced a dose- and time-related increase in the mass of Ins(1,4,5)P3 (basal 38.3 +/- 5.8 pmol/mg of protein) and Ins(1,3,4,5)P4 (basal 6.1 +/- 1.2 pmol/mg of protein). Comparison of radioreceptor mass assays with [3H]inositol labelling showed higher-fold stimulations with the former protocol. The later accumulation of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass was dependent upon extracellular Ca2+.
摘要
由SH-SY5Y细胞表达的M3毒蕈碱受体的刺激导致Ins(1,4,5)P3（基础值为38.3±5.8 pmol/mg蛋白质）和Ins(1,3,4,5)P4（基础值为6.1±1.2 pmol/mg蛋白质）的量呈剂量和时间依赖性增加。放射性受体质量测定与[3H]肌醇标记的比较显示，前一种方案的刺激倍数更高。Ins(1,4,5)P3和Ins(1,3,4,5)P4量的后期积累依赖于细胞外Ca2+。
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