培养的大鼠肝细胞中胆汁酸合成及胆固醇7α-羟化酶活性的维持
Maintenance of bile acid synthesis and cholesterol 7 alpha-hydroxylase activity in cultured rat hepatocytes.
作者信息
Princen H M, Meijer P
机构信息
Gaubius Institute TNO, Leiden, The Netherlands.
出版信息
Biochem J. 1990 Nov 15;272(1):273-5. doi: 10.1042/bj2720273.
Abstract
Addition of foetal-bovine serum to rat hepatocytes cultured in Williams E medium resulted in improved maintenance of bile-acid-synthetic capacity and cholesterol 7 alpha-hydroxylase activity as compared with cultures supplemented with rat or newborn-bovine serum or cultures in a hormonally defined serum-free medium. Minimally, 5% (v/v) foetal-bovine serum was necessary to maintain these liver-specific functions. Serum factor(s) responsible for these effects were not dialysable or associated with lipoproteins, but were removed by charcoal extraction.
摘要
与添加大鼠血清、新生牛血清的培养物或激素限定的无血清培养基中的培养物相比，向在Williams E培养基中培养的大鼠肝细胞中添加胎牛血清可改善胆汁酸合成能力和胆固醇7α-羟化酶活性的维持。维持这些肝脏特异性功能至少需要5%（v/v）的胎牛血清。负责这些作用的血清因子不可透析，也不与脂蛋白相关，但可通过活性炭提取去除。
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