Cell Biology and Immunology Group, Wageningen University and Research Centre, Wageningen, The Netherlands.
Mol Nutr Food Res. 2012 May;56(5):822-33. doi: 10.1002/mnfr.201100715.
We aimed to examine different immunological aspects of β-glucans derived from different food sources (oat, barley and shiitake) on phorbol myristate acetate (PMA)-differentiated THP-1 macrophages. Commercially purified barley β-glucan (commercial BG) and lentinan were included to compare β-glucans from the same origin but different degree of purity and processing.
Chemical composition and molecular weight distribution of β-glucan samples were determined. Inflammation-related gene expression kinetics (IL-1β, IL-8, nuclear factor kappa B [NF-κB] and IL-10) after 3, 6 and 24 h of stimulation with 100 μg/mL β-glucan were investigated. All tested β-glucans mildly upregulated the observed inflammation-related genes with differential gene expression patterns. Similar gene expression kinetics, but different fold induction values, was found for the crude β-glucan extracts and their corresponding commercial forms. Pre-incubation of THP-1 macrophages with β-glucans prior to lipopolysaccharide (LPS) exposure decreased the induction of inflammation-related genes compared to LPS treatment. No production of nitric oxide (NO) and hydrogen peroxide (H₂O₂) was detected in β-glucan stimulated THP-1 macrophages. Phagocytic activity was not different after stimulation by β-glucan samples.
Based on these in vitro analyses, it can be concluded that the analysed β-glucans have varying levels of immunomodulating properties, which are likely related to structure, molecular weight and compositional characteristic of β-glucan.
本研究旨在考察不同来源（燕麦、大麦和香菇）β-葡聚糖在佛波醇肉豆蔻酸酯（PMA）诱导的 THP-1 巨噬细胞中的不同免疫学特性。同时纳入了商业上纯化的大麦β-葡聚糖（商业 BG）和香菇多糖，以比较来源相同但纯度和加工方式不同的β-葡聚糖。
对β-葡聚糖样品的化学组成和分子量分布进行了测定。在 100μg/mLβ-葡聚糖刺激 3、6 和 24 小时后，研究了与炎症相关的基因表达动力学（IL-1β、IL-8、核因子 kappa B[NF-κB]和 IL-10）。所有测试的β-葡聚糖均轻度上调了观察到的与炎症相关的基因，表现出不同的基因表达模式。粗β-葡聚糖提取物及其相应的商业形式具有相似的基因表达动力学，但诱导倍数不同。与 LPS 处理相比，在 LPS 暴露前用β-葡聚糖预孵育 THP-1 巨噬细胞可降低与炎症相关的基因的诱导。在β-葡聚糖刺激的 THP-1 巨噬细胞中未检测到一氧化氮（NO）和过氧化氢（H₂O₂）的产生。β-葡聚糖样品刺激后，吞噬活性没有差异。
基于这些体外分析，可以得出结论，所分析的β-葡聚糖具有不同水平的免疫调节特性，这可能与β-葡聚糖的结构、分子量和组成特征有关。
