Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Carbohydr Res. 2010 Sep 3;345(13):1851-5. doi: 10.1016/j.carres.2010.06.006. Epub 2010 Jun 20.
This study evaluated the effect of phosphorylated glucosamine (pGlc) on the regulation of cytokines involved in immunological activities. Changes in the inflammatory profiles of lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were investigated following pGlc treatment. Treatment with pGlc inhibited the production of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6). In addition, pGlc suppressed the regulation of inflammatory mediators such as TNF-alpha, IL-1beta, IL-6, inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) in LPS-stimulated THP-1 macrophages. Furthermore, we confirmed that the LPS-stimulated transcription of MAP kinases in PMA-differentiated THP-1 macrophages was inhibited by pGlc. According to this study, pGlc can be considered as a potential anti-inflammatory agent.
本研究评估了磷酸化葡萄糖胺（pGlc）对参与免疫活性的细胞因子调节的影响。在 pGlc 处理后，研究了磷酸化葡萄糖胺处理对脂多糖（LPS）刺激佛波醇 12-肉豆蔻酸 13-醋酸酯（PMA）分化的 THP-1 巨噬细胞模型中炎症谱的变化。pGlc 抑制了促炎细胞因子如肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的产生。此外，pGlc 抑制了 LPS 刺激的 THP-1 巨噬细胞中炎症介质如 TNF-α、IL-1β、IL-6、诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的调节。此外，我们证实 pGlc 抑制了 LPS 刺激的 PMA 分化的 THP-1 巨噬细胞中 MAP 激酶的转录。根据这项研究，pGlc 可以被认为是一种潜在的抗炎剂。
