噬菌体展示技术在丝状噬菌体基础上的应用：用于重组抗体的选择。
Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection.
机构信息
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Science.
出版信息
Acta Naturae. 2009 Oct;1(3):20-8.
Abstract
The display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DNA fragment exist in one phage particle. Development of phage display antibody libraries provides repertoires of phage particles exposing antibody fragments of great diversity. The biopanning procedure facilitates selection of antibodies with high affinity and specificity for almost any target. This review is an introduction to phage display methodology. It presents recombinant antibodies display in more details:, construction of phage libraries of antibody fragments and different strategies for the biopanning procedure.
摘要
丝状噬菌体表面展示肽和蛋白质是一种用于选择肽和蛋白质结构域（包括抗体）的强大方法。这种方法的一个优点是表型和基因型之间的直接物理联系，因为分析的多肽及其编码 DNA 片段存在于一个噬菌体颗粒中。噬菌体展示抗体文库的开发提供了展示多样性极大的噬菌体颗粒的抗体片段库。生物淘选程序有利于选择对几乎任何目标具有高亲和力和特异性的抗体。本文是对噬菌体展示方法的介绍。它更详细地介绍了重组抗体的展示：抗体片段的噬菌体文库的构建以及生物淘选程序的不同策略。
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