寻找与 SARS-CoV-2 表面蛋白受体结合域相互作用的单域抗体。
The Search for Single-Domain Antibodies Interacting with the Receptor-Binding Domain of SARS-CoV-2 Surface Protein.
机构信息
State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.
Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Bull Exp Biol Med. 2023 Jun;175(2):225-228. doi: 10.1007/s10517-023-05839-6. Epub 2023 Jul 19.
We performed a search for nanoantibodies that specifically interact with the receptor-binding domain (RBD) of the SARS-CoV-2 surface protein. The specificity of single-domain antibodies from the blood sera of a llama immunized with RBD of SARS-CoV-2 surface protein S (variant B.1.1.7 (Alpha)) was analyzed by ELISA. Recombinant trimers of the SARS-CoV-2 spike protein were used as antigens. In this work, a set of single-domain antibodies was obtained that specifically bind to the RBD of the SARS-CoV-2 virus.
我们搜索了能够特异性结合 SARS-CoV-2 表面蛋白受体结合域（RBD）的纳米抗体。通过 ELISA 分析了用 SARS-CoV-2 表面蛋白 S（变体 B.1.1.7（Alpha））的 RBD 免疫的骆驼血清中的单域抗体的特异性。使用 SARS-CoV-2 刺突蛋白的重组三聚体作为抗原。在这项工作中，获得了一组能够特异性结合 SARS-CoV-2 病毒 RBD 的单域抗体。
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