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哺乳动物细胞中 5-甲基胞嘧啶残基的脱氨作用。

Deamination of 5-methylcytosine residues in Mammalian cells.

机构信息

Department of Chemistry, Lomonosov Moscow State University;

出版信息

Acta Naturae. 2009 Oct;1(3):121-4.

PMID:22649624
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3347525/
Abstract

DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are treated with nuclear extracts from the cell lines CHO, HeLa, and Skov3. The hypothesis that deamination of 5-methylcytosine is the first stage of demethylation in mammalia has been postulated.

摘要

哺乳动物中的 DNA 去甲基化发生在受精后和胚胎发生过程中，并伴随着细胞衰老和癌症转化。借助引物延伸反应、MALDI MS 和胸腺嘧啶 DNA 糖基化酶的 DNA 切割，当用来自 CHO、HeLa 和 Skov3 细胞系的核提取物处理模型甲基化 DNA 双链体时，已经显示出 5-甲基胞嘧啶残基的脱氨作用发生。已经提出了 5-甲基胞嘧啶脱氨作用是哺乳动物中去甲基化的第一阶段的假说。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/343856e5bfe1/AN20758251-03-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/fc347c3aa7c1/AN20758251-03-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/dd7db7ad141f/AN20758251-03-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/343856e5bfe1/AN20758251-03-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/fc347c3aa7c1/AN20758251-03-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/dd7db7ad141f/AN20758251-03-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af37/3347525/343856e5bfe1/AN20758251-03-121-g003.jpg

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本文引用的文献

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DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45.斑马鱼中的DNA去甲基化涉及一种脱氨酶、一种糖基化酶和生长停滞及DNA损伤诱导蛋白45（Gadd45）的偶联。
Cell. 2008 Dec 26;135(7):1201-12. doi: 10.1016/j.cell.2008.11.042.
2
Cyclical DNA methylation of a transcriptionally active promoter.转录活性启动子的周期性DNA甲基化。
Nature. 2008 Mar 6;452(7183):45-50. doi: 10.1038/nature06544.
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DNA demethylation in the Arabidopsis genome.拟南芥基因组中的DNA去甲基化
Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6752-7. doi: 10.1073/pnas.0701861104. Epub 2007 Apr 4.
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Covalent modification of DNA regulates memory formation.DNA的共价修饰调节记忆形成。
Neuron. 2007 Mar 15;53(6):857-69. doi: 10.1016/j.neuron.2007.02.022.
5
Non-heme dioxygenases: cellular sensors and regulators jelly rolled into one?非血红素双加氧酶：兼具细胞传感器与调节因子功能？
Nat Chem Biol. 2007 Mar;3(3):144-53. doi: 10.1038/nchembio863.
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Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.Gadd45a通过修复介导的DNA去甲基化促进表观遗传基因激活。
Nature. 2007 Feb 8;445(7128):671-5. doi: 10.1038/nature05515. Epub 2007 Jan 31.
7
The enigmatic thymine DNA glycosylase.神秘的胸腺嘧啶DNA糖基化酶。
DNA Repair (Amst). 2007 Apr 1;6(4):489-504. doi: 10.1016/j.dnarep.2006.10.013. Epub 2006 Nov 20.
8
DNA hypomethylation and human diseases.DNA低甲基化与人类疾病
Biochim Biophys Acta. 2007 Jan;1775(1):138-62. doi: 10.1016/j.bbcan.2006.08.007. Epub 2006 Sep 1.
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J Immunol. 2006 Jul 15;177(2):1062-9. doi: 10.4049/jimmunol.177.2.1062.
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DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases.去甲基化酶（DEMETER）和沉默抑制因子1（REPRESSOR OF SILENCING 1）编码5-甲基胞嘧啶DNA糖基化酶。
Proc Natl Acad Sci U S A. 2006 May 2;103(18):6853-8. doi: 10.1073/pnas.0601109103. Epub 2006 Apr 19.