Belova E V, Kozlov A E, Shubernetskaya O S, Zvereva M I, Shpanchenko O V, Dontsova O A
Chemical Department, Moscow State University, Moscow, 119991, Russia.
Belozerskii Institute of Physicochemical Biology, Moscow State University, Moscow, 119991, Russia.
Dokl Biochem Biophys. 2015;464:329-32. doi: 10.1134/S1607672915050166. Epub 2015 Oct 31.
The methylation status of the genes of telomerase reverse transcriptase (tert) and telomerase RNA (terc) was determined in brain tissues of Danio rerio of different age. It is found that, regardless of the age of fish, the regulatory region of the tert gene was completely methylated, whereas the coding region remained unmethylated in all cases. The level of methylation of the region located downstream of the coding region of the terc gene changes with age. This region was analyzed in the samples of other tissues, and its methylation status was also nonuniform. The alteration of the methylation status in the 3'-untranslated region of the terc gene suggests the possibility of transcription of the antisense strand in this region.
测定了不同年龄斑马鱼脑组织中端粒酶逆转录酶（tert）和端粒酶RNA（terc）基因的甲基化状态。结果发现，无论鱼的年龄如何，tert基因的调控区域均完全甲基化，而编码区域在所有情况下均保持未甲基化状态。terc基因编码区域下游区域的甲基化水平随年龄变化。在其他组织样本中对该区域进行了分析，其甲基化状态也不一致。terc基因3'-非翻译区甲基化状态的改变提示该区域存在反义链转录的可能性。
