Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences.
Acta Naturae. 2011 Jan;3(1):77-84.
Pattern-recognition receptors (PRR) play a crucial role in the induction of the defense reactions of the immune system against pathogenic bacterial and viral infections. The activation of PRR by specific, highly conserved pathogen-associated molecular patterns (PAMPs) induces numerous immune reactions related both to innate and adaptive immunity. In addition to the well-studied Toll-like receptors, pathogens can be recognized by the receptors belonging to the other PRR families; including NOD-like receptors (NLR). Stimulation of members of NOD-like receptors (NOD1, 2) and Toll-like receptors results in the activation of the transcriptional factor NF-kB regulating gene expression in numerous molecules implicated in the development of proinflammatory reactions. As opposed to Toll-like receptors, the NF-kB-activating ability of NLRs has not been fully studied. In this work, we examine the ability of one member of the NLR family - NOD1 - to activate the main proinflammatory transcriptional factor NF-kB. We also compare the NF-kB-activating ability of NOD1 ligands of a different structure with TLR4,5 ligandsin vitroandin vivo.
模式识别受体 (PRR) 在诱导免疫系统对致病性细菌和病毒感染的防御反应中起着至关重要的作用。PRR 被特定的、高度保守的病原体相关分子模式 (PAMP) 的激活，诱导与先天免疫和适应性免疫相关的许多免疫反应。除了研究得很好的 Toll 样受体外，病原体还可以被属于其他 PRR 家族的受体识别; 包括 NOD 样受体 (NLR)。NOD 样受体 (NOD1、2) 和 Toll 样受体的刺激导致转录因子 NF-kB 的激活，调节参与炎症反应发展的众多分子的基因表达。与 Toll 样受体不同，NLRs 的 NF-kB 激活能力尚未得到充分研究。在这项工作中，我们研究了 NLR 家族的一个成员 NOD1 激活主要促炎转录因子 NF-kB 的能力。我们还比较了不同结构的 NOD1 配体与 TLR4、5 配体在体外和体内的 NF-kB 激活能力。
