Xue Hong-Li, Huang Xue-Di, He Dong, Lin Shi-Jia, Wang Shu, Niu Ting
Department of Hematology & Research Laboratory of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Mar;43(2):174-9.
To investigate the effects of the extract from Marsdensia tenacissima on proliferation and apoptosis of human hematologic neoplasm cell line cells.
Raji, NB4 and K562 cells were treated in vitro with different concentrations of the extract from Marsdensia tenacissima, including different ethanol elution components and C21 steroidal saponin monomer compounds, for different periods. Tumor cell proliferation was measured by MTT colorimetric assay and its apoptosis was determined by the flow cytometry (FCM).
Firstly, with higher concentrations, 100 microg/mL and 200 microg/mL, 70% ethanol eluate from Marsdensia tenacissima inhibited the proliferation of Raji, NB4 and K562 cells significantly, in a dose and time dependent manner, compared with 30% and 50% ethanol elution components from Marsdensia tenacissima (P < 0.05). Secondly, four C21 steroidal saponin monomer compounds, tenacissosides B,C,I and marsdenoside K, also inhibited the proliferation of Raji, NB4 and K562 cells in vitro significantly, in a dose and time dependent manner, compared with that of control group (P < 0.05). Among them, tenacissoside C showed the strongest inhibition effects on proliferation of these cells under all experimental conditions compared with the other three C21 steroidal saponin monomer compounds (P < 0.05). Furthermor, the IC50 of tenacissosides C on proliferation of Raji, NB4 and K562 cells were 64.1 micromol/L, 70.4 micromol/L and 105.8 micromol/L, respectively. Finally, after Raji, NB4 and K562 cells were treated with 98.4 micromol/L tenacissoside C for 24 h and 48 h, the early apoptosis rates and late apoptosis rates of these tumor cells increased markedly, compared with the control group (P < 0.05).
The extract from Marsdensia tenacissima, including different ethanol elution components and C21 steroidal saponin monomer compounds, may inhibit the proliferation of some human hematologic neoplasm cell line cells and induce these tumor cells apoptosis in vitro, especially tenacissoside C, one of the C21 steroidal saponin monomer compounds, showed the strongest effects on proliferation of these tumor cells when compared with other ones, with the strongest inhibition activities on human Burkitt's lymphoma cell line Raji cells.
探讨通光藤提取物对人血液系统肿瘤细胞株细胞增殖及凋亡的影响。
采用不同浓度的通光藤提取物（包括不同乙醇洗脱成分及C21甾体皂苷单体化合物）体外处理Raji、NB4和K562细胞不同时间。采用MTT比色法检测肿瘤细胞增殖，流式细胞术（FCM）检测其凋亡情况。
首先，通光藤70%乙醇洗脱物在100μg/mL和200μg/mL较高浓度时，能显著抑制Raji、NB4和K562细胞增殖，呈剂量和时间依赖性，与通光藤30%和50%乙醇洗脱成分相比（P<0.05）。其次，4种C21甾体皂苷单体化合物，即通光藤苷B、C、I和通光藤新苷K，也能显著抑制Raji、NB4和K562细胞体外增殖，呈剂量和时间依赖性，与对照组相比（P<0.05）。其中，通光藤苷C在所有实验条件下对这些细胞增殖的抑制作用最强，与其他3种C21甾体皂苷单体化合物相比（P<0.05）。此外，通光藤苷C对Raji、NB4和K562细胞增殖的IC50分别为64.1μmol/L、70.4μmol/L和105.8μmol/L。最后，Raji、NB4和K562细胞经98.4μmol/L通光藤苷C处理24小时和48小时后，这些肿瘤细胞的早期凋亡率和晚期凋亡率均明显升高，与对照组相比（P<0.05）。
通光藤提取物，包括不同乙醇洗脱成分及C21甾体皂苷单体化合物，可能在体外抑制某些人血液系统肿瘤细胞株细胞的增殖并诱导这些肿瘤细胞凋亡，尤其是C21甾体皂苷单体化合物之一的通光藤苷C，与其他化合物相比，对这些肿瘤细胞增殖的影响最强，对人伯基特淋巴瘤细胞株Raji细胞的抑制活性最强。
