Department of Gynecology, The No. 2 Hospital of Baoding, 071000, Baoding, Hebei, China.
Department of Gynecology, The General Hospital of Ningxia Medical University, 750004, Yinchuan, Ningxia, China.
Dokl Biochem Biophys. 2021 Mar;497(1):144-150. doi: 10.1134/S1607672921020058. Epub 2021 Apr 24.
Ovarian cancer is the malignant tumour of the female reproductive organ with highest mortality rate among all the types of gynaecological tumours. This study investigated the effect of Dioscorea deltoidea leaf extract (DDLE) on OV-90 and CAOV4 ovarian cancer cells. The results demonstrated that DDLE suppresses OV-90 and CAOV3 cell viability significantly in dose dependent manner. The OV-90 and CAOV3 cell viability were reduced to 24 and 27% respectively with 20 mg/mL DDLE treatment. Five mg/mL DDLE treatment of OV-90 and CAOV4 cells raised percentage of cells in G2-phase to 55.9 and 51.2%, respectively. In 5 mg/mL DDLE -treated OV-90 and CAOV4 cells a prominent suppression in cyclin-D1 and cyclin B1 proteins was observed in 48 h. The DDLE treatment promoted OV-90 and CAOV3 cell apoptosis to 34.65 and 29.89%, respectively. The Fas, FasL, cleaved caspase-3, and Bax levels were up-regulated markedly in the cells after DDLE treatment. Moreover, DDLE treatment suppressed p-mTOR, p-AKT and p-PI3K expression in OV-90 and CAOV3 cells. Thus, DDLE suppressed ovary cancer cell viability and elevated cell apoptosis. Inhibitory effect of DDLE on ovarian cancer cells is associated with targeting PI3K/AKT/mTOR pathway.
卵巢癌是女性生殖器官恶性肿瘤中死亡率最高的肿瘤。本研究探讨了薯蓣叶提取物（DDLE）对卵巢癌细胞（OV-90 和 CAOV4）的影响。结果表明，DDLE 呈剂量依赖性显著抑制 OV-90 和 CAOV3 细胞活力。用 20mg/mL DDLE 处理后，OV-90 和 CAOV3 细胞活力分别降至 24%和 27%。用 5mg/mL DDLE 处理 OV-90 和 CAOV4 细胞可将细胞周期 G2 期的百分比分别提高到 55.9%和 51.2%。在 5mg/mL DDLE 处理的 OV-90 和 CAOV4 细胞中，细胞周期蛋白 D1 和细胞周期蛋白 B1 蛋白在 48 小时内明显受到抑制。DDLE 处理可将 OV-90 和 CAOV3 细胞凋亡率分别提高至 34.65%和 29.89%。Fas、FasL、cleaved caspase-3 和 Bax 水平在 DDLE 处理后细胞中明显上调。此外，DDLE 处理抑制了 OV-90 和 CAOV3 细胞中 p-mTOR、p-AKT 和 p-PI3K 的表达。因此，DDLE 抑制卵巢癌细胞活力并提高细胞凋亡。DDLE 对卵巢癌细胞的抑制作用与靶向 PI3K/AKT/mTOR 通路有关。
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