["双固一通”方电针治疗对衰老大鼠T淋巴细胞亚群比例的影响]

[Effects of electroacupuncture of "Shuanggu Yitong" prescription on the T lymphocyte subset proportions in aging rats].

作者信息

Xiao Ling, Wang Guang-An, Wang Hua

机构信息

College of Acupuncture and Orthopedics, Hubei University of CM, Wuhan 430061, Hubei Province, China.

出版信息

Zhongguo Zhen Jiu. 2012 May;32(5):435-9.

PMID:22650133

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture of "Shuanggu Yitong" prescription on postponing aging.

METHODS

Forty 3-month SD rats, male only, 30 rats were made sub-acute aging model by D-galactose s.c. injection continuously for 42 d, and rest of the rats, 10, were divided into a normal control group. After the modeling, the sub-acute aging model rats were randomly into a Shuanggu Yitong group [electroacupuncture at "Guanyuan" (CV 4) and "Zusanli" (ST 36), hand needle at "Baihui" (GV 20)], an acupuncture control group [electroacupuncture at "Weizhong" (BL 40) and "Shuifen" (CV 9), hand needle at "Yintang" (GV 29)] and an aging model group, ten in each one. The treatment was given once in a day, six of which made a course. The rats in the normal control group and aging model group were not received any treatment. After the treatment for three weeks, the rats were put to death and their spleen index, thymus index and the T lymphocytes subgroups (CD8(+) T/T cell and CD8(+) CD28(-) T/CD8(+) T cell) were tested.

RESULTS

The spleen index (1.74 +/- 0.059) and thymus index (0.64 +/- 0.039) in the aging model group was obviously lower than those in the normal control group (1.93 +/- 0.061), (0.81 +/- 0.053) respectively (both P < 0.05); the CD8(+) CD28(-) T/CD8(+) T cell percentages (26.28 +/- 4.69)% and CD8(+) T/T cell percentages (43.33 +/- 2.84)% in the aging model group were both significantly higher than those (15.08 +/- 5.58)% (P < 0.01), (34.70 +/- 4.24)% (P < 0.01) in the normal control group. Compared with the aging model group, the spleen index (1.91 +/- 0.081) and thymus index (0.79 +/- 0.080) in the Shuanggu Yitong group were significantly higher (both P < 0.05), but obviously decreased with the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73) (P < 0.01); the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73)% in the acupuncture control group was also lower than the aging model group (P < 0.05), but more obvious reduce for the Shuanggu Yitong group (P < 0.05).

CONCLUSION

The treatment of Shuanggu Yitong prescription could regulate the proportions of the T lymphocyte subset, and slow down the immunosenescence of subacute aging model rats induced by D-galactose.

摘要

目的

探讨“双固一通”方电针延缓衰老的机制。

方法

40只3月龄雄性SD大鼠，30只通过连续皮下注射D-半乳糖42天制备亚急性衰老模型，其余10只作为正常对照组。造模后，将亚急性衰老模型大鼠随机分为双固一通组[电针“关元”（CV4）、“足三里”（ST36），手针“百会”（GV20）]、针刺对照组[电针“委中”（BL40）、“水分”（CV9），手针“印堂”（GV29）]和衰老模型组，每组10只。每日治疗1次，6次为1个疗程。正常对照组和衰老模型组大鼠不接受任何治疗。治疗3周后处死大鼠，检测其脾脏指数、胸腺指数及T淋巴细胞亚群（CD8(+)T/T细胞和CD8(+)CD28(-)T/CD8(+)T细胞）。

结果

衰老模型组脾脏指数（1.74±0.059）和胸腺指数（0.64±0.039）明显低于正常对照组（分别为1.93±0.061、0.81±0.053）（均P<0.05）；衰老模型组CD8(+)CD28(-)T/CD8(+)T细胞百分比（26.28±4.69）%和CD8(+)T/T细胞百分比（43.33±2.84）%均显著高于正常对照组（分别为15.08±5.58）%（P<0.01）、（34.70±4.24）%（P<0.01）。与衰老模型组比较，双固一通组脾脏指数（1.91±0.081）和胸腺指数（0.79±0.080）显著升高（均P<0.05），而CD8(+)CD28(-)T/CD8(+)T细胞百分比（18.07±1.73）%明显降低（P<0.01）；针刺对照组CD8(+)CD28(-)T/CD8(+)T细胞百分比（18.07±1.73）%也低于衰老模型组（P<0.05），但双固一通组降低更明显（P<0.05）。