Liu Jianmin, Liu Jing, Wang Guang'an, Liu Guangya, Zhou Huanjiao, Fan Yun, Liang Fengxia, Wang Hua
Acupuncture-moxibustion and Orthopedic College, Hubei University of Chinese Medicine, Wuhan, China.
Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture & Moxibustion, Wuhan, China.
PLoS One. 2018 Jan 19;13(1):e0191623. doi: 10.1371/journal.pone.0191623. eCollection 2018.
To investigate the molecular mechanisms of sub-acutely aging and demonstrate the effect of electroacupuncture (EA) at the Guanyuan (CV 4), Zusanli (ST 36) and Baihui (DU 20) acupoint on the sub-acutely aging brain, cDNA microarrays and bioinformatics analyses were carried out. Thirty Sprague-Dawley (SD) male rats were selected and randomly divided into three groups: the control group (C), the sub-acutely aging model group (M) and the electroacupuncture group (M+EA). Sub-acutely aging model rats were obtained by D-galactose s.c. injection continuously for 40 days. Total RNA was extracted from the hippocampus area of brains in three groups for cDNA microarrays. The data of different groups were compared and analyzed by differential expression analysis, Gene ontology (GO) term enrichment, Kyoto Encyclopedia of Genes Genomes (KEGG) pathway enrichment and quantitative real-time PCR. According to the results, 4052 DE genes were identified in our study. Among them, there were 3079 differentially expressed (DE) genes between group M and group C, and these genes are associated with the aging of rats. Moreover, 983 genes were expressed differently in group M+EA compared with group M, revealing that points stimuli could regulate gene expression in brain with aging. Gene ontology (GO) term enrichment and KEGG enrichment were performed to further classify the differential expression genes. Important GO terms and KEGG pathways connected with sub-acutely aging EA effects were identified. At last, 3 significant differentially expressed genes were selected for real-time quantitative PCR to clarify the cDNA microarray results. In conclusion, the cDNA microarray data first compared and analyzed the differences of gene expression profile in the hippocampus of rats in different groups, which contribute to our knowledge on the molecular mechanisms of EA towards sub-acutely aging.
为探讨亚急性衰老的分子机制，并阐明电针关元（CV 4）、足三里（ST 36）和百会（DU 20）穴位对亚急性衰老大脑的影响，进行了cDNA微阵列和生物信息学分析。选取30只雄性Sprague-Dawley（SD）大鼠，随机分为三组：对照组（C）、亚急性衰老模型组（M）和电针组（M+EA）。通过连续40天皮下注射D-半乳糖获得亚急性衰老模型大鼠。从三组大鼠大脑的海马区提取总RNA用于cDNA微阵列分析。通过差异表达分析、基因本体（GO）术语富集、京都基因与基因组百科全书（KEGG）通路富集和定量实时PCR对不同组的数据进行比较和分析。根据结果，本研究共鉴定出4052个差异表达基因（DE基因）。其中，M组和C组之间有3079个差异表达基因，这些基因与大鼠衰老相关。此外，与M组相比，M+EA组有983个基因表达不同，表明穴位刺激可调节衰老大脑中的基因表达。进行基因本体（GO）术语富集和KEGG富集以进一步对差异表达基因进行分类。确定了与亚急性衰老电针效应相关的重要GO术语和KEGG通路。最后，选择3个显著差异表达基因进行实时定量PCR以阐明cDNA微阵列结果。总之，cDNA微阵列数据首次比较并分析了不同组大鼠海马中基因表达谱的差异，这有助于我们了解电针对亚急性衰老的分子机制。
