Zagury J F, Josephs S F, Agius G, Nicol I, Willer A, Kalyanaraman V S, Zagury D, Wong-Staal F, Gallo R C
Universite Pierre et Marie Curie, Paris, France.
AIDS Res Hum Retroviruses. 1990 Sep;6(9):1079-85. doi: 10.1089/aid.1990.6.1079.
We have previously described the cloning and sequencing of a novel stain of human immunodeficiency virus type 2 (HIV-2) called HIV-2NIH-Z. A plasmid clone, pHIV2Z, containing the full-length provirus has now been constructed, and virus particles have been obtained upon transfection into COS-1 and H-9 cells. These particles can infect a number of T-cell lines and exert a cytopathic effect on fresh human and macaque peripheral blood lymphocytes. The cloned virus is biologically and morphologically indistinguishable from its parental uncloned strain as shown by restriction enzyme analysis, electron microscopy, and kinetics of infection. However, as shown by radioimmunoprecipitation assays, the cloned virus-infected cells express a full-length gp41 protein as predicted by the nucleotide sequence, whereas the wild-type parental strain expresses a truncated gp33 protein. Both the parental strain and the cloned virus possess a deletion encompassing the end of the nef gene within the U3 region which apparently does not affect their in vitro cytopathic and replicative capacities.
我们之前描述了一种名为HIV-2NIH-Z的新型2型人类免疫缺陷病毒（HIV-2）毒株的克隆和测序。现在构建了一个包含全长前病毒的质粒克隆pHIV2Z，并在转染到COS-1和H-9细胞后获得了病毒颗粒。这些颗粒可以感染多种T细胞系，并对新鲜的人类和猕猴外周血淋巴细胞产生细胞病变效应。如通过限制性内切酶分析、电子显微镜检查和感染动力学所示，克隆病毒在生物学和形态学上与其未克隆的亲代毒株没有区别。然而，如放射免疫沉淀试验所示，克隆病毒感染的细胞表达了核苷酸序列预测的全长gp41蛋白，而野生型亲代毒株表达的是截短的gp33蛋白。亲代毒株和克隆病毒都在U3区域内有一个包含nef基因末端的缺失，这显然不影响它们的体外细胞病变和复制能力。
