Rojo David, Barbas Coral, Rupérez Francisco J
Center for Metabolomics & Bioanalysis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo, Campus Montepríncipe, Boadilla del Monte, Madrid 28668, Spain.
Bioanalysis. 2012 Jun;4(10):1235-43. doi: 10.4155/bio.12.100.
The metabolome is the complete set of small molecules coming from protein activity (anabolism and catabolism) in living systems. They have a broad range of chemical structures and physicochemical properties and therefore different analytical methodologies are necessary. Highly polar metabolites, such as sugars and most amino acids are not retained by conventional reversed-phase LC columns. Without sufficient retention, coelution may result in identification problems while the detection of compounds by MS at low concentrations may also be problematic due to ion suppression. In order to retain compounds based on their hydrophilicity, polar stationary phases and hydrophilic-interaction LC provide a complementary tool to reversed-phase LC for untargeted comprehensive metabolite fingerprinting. However, robustness of the methods is still limiting their applications. This review focuses on sample pretreatment, stationary phases, analytical methods and applications for polar compound analysis in biological matrices.
代谢组是指来自生物系统中蛋白质活性（合成代谢和分解代谢）的所有小分子的集合。它们具有广泛的化学结构和物理化学性质，因此需要不同的分析方法。高极性代谢物，如糖类和大多数氨基酸，不能被传统的反相液相色谱柱保留。如果没有足够的保留，共洗脱可能会导致鉴定问题，而在低浓度下通过质谱检测化合物也可能因离子抑制而存在问题。为了根据化合物的亲水性进行保留，极性固定相和亲水相互作用液相色谱为反相液相色谱提供了一种补充工具，用于非靶向全面代谢物指纹图谱分析。然而，这些方法的稳健性仍然限制了它们的应用。本综述重点关注生物基质中极性化合物分析的样品预处理、固定相、分析方法及应用。
