Department of Biological Sciences, Birla Institute of Technology and Science-Pilani, K.K. Birla Goa Campus, Zuarinagar, Goa 403726, India.
Toxicon. 2012 Oct;60(5):719-23. doi: 10.1016/j.toxicon.2012.05.008. Epub 2012 May 28.
Moon jellyfish (Aurelia aurita) tentacle extract was studied for its anticoagulant activity in vitro. The Jellyfish Tentacle Extract (JFTE) showed very strong fibrinogenolytic activity by cleaving Aα and Bβ chain of fibrinogen molecule. The fibrinogenolytic activity was found to be stronger than some snake venom derived anticoagulants. JFTE also completely liquefied fibrin clots in 24 h. JFTE was found to contain both high and low molecular weight proteins/peptides. The fibrinogenolysis appears to be caused by high molecular weight fractions of the extract. It has been also noted that PMSF significantly reduced fibrinogenolytic activity and heating totally abolished it. Autolytic degradation of the high molecular weight protein was also noted. Autolysis slowed down, but did not abolish the fibrinogenolytic activity of the extract.
海月水母（Aurelia aurita）触手提取物的体外抗凝活性研究。水母触手提取物（JFTE）通过裂解纤维蛋白分子的 Aα 和 Bβ 链表现出很强的纤维蛋白原水解活性。该纤维蛋白原水解活性比一些蛇毒来源的抗凝剂更强。JFTE 还能在 24 小时内完全溶解纤维蛋白凝块。JFTE 被发现含有高分子量和低分子量的蛋白质/肽。纤维蛋白原水解似乎是由提取物的高分子量部分引起的。研究还注意到，PMSF 显著降低了纤维蛋白原水解活性，而加热则完全消除了该活性。高分子量蛋白质的自溶降解也被注意到。自溶降解速度减慢，但没有完全消除提取物的纤维蛋白原水解活性。
