Prabhakar A R, Sharma D, Sugandhan S
Department of Pedodontics and Preventive Dentistry, Bapuji Dental College and Hospital, Karnataka, India.
Eur Arch Paediatr Dent. 2012 Jun;13(3):138-43. doi: 10.1007/BF03262860.
To evaluate and compare in vitro the remineralising effects and surface microhardness of glass ionomer cement (GIC), GIC containing grape seed extract and GIC containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP).
An in vitro study with 45 mandibular premolars with standardised class V cavities prepared on the buccal and lingual surfaces. Artificial caries-like lesions were created and teeth were randomly divided into 3 groups of 15 teeth each. Each tooth was sectioned longitudinally into buccal and lingual halves; lingual half was used as control and the other as the test specimen. Axial longitudinal sections of the control specimens were analysed under polarised light microscopy. The experimental halves were restored with 3 materials. Group I: glass ionomer cement (GIC), Group II: 10% (w/w) grape seed extract and GIC (GSGIC), Group III: 10% (w/w) casein phosphopeptide-amorphous calcium phosphate (Recaldent) and GIC (CPPGIC). The restored tooth specimens were exposed to pH cycling for 28 days and analysed again for remineralisation under polarised microscopy. For the second part of the study, 60 cylindrical specimens (20 each) were made using standardised brass moulds and the three experimental materials. The specimens were immersed individually in de-ionised water. After 7 days of immersion, 10 specimens from each group were subjected to microhardness measurements and repeated on the remaining specimens at the end of 30 days.
One way ANOVA and post-hoc Tukey's test were used for statistical analysis.
CPPGIC showed the greatest remineralisation followed by GSGIC and least for GIC. Comparisons of microhardness between the three groups were not statistically significant.
Grape seed extract and CPPACP can be used for remineralisation of damaged dentine and their incorporation into GIC does not compromise the microhardness properties significantly.
在体外评估并比较玻璃离子水门汀（GIC）、含葡萄籽提取物的GIC以及含酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）的GIC的再矿化效果和表面显微硬度。
一项体外研究，使用45颗下颌前磨牙，在其颊面和舌面制备标准化的V类洞。制造人工龋样病变，牙齿被随机分为3组，每组15颗牙。每颗牙齿纵向切成颊侧和舌侧两半；舌侧半用作对照，另一半用作测试标本。对照标本的轴向纵切片在偏光显微镜下进行分析。实验半片用3种材料修复。第一组：玻璃离子水门汀（GIC），第二组：10%（w/w）葡萄籽提取物与GIC（GSGIC），第三组：10%（w/w）酪蛋白磷酸肽-无定形磷酸钙（Recaldent）与GIC（CPPGIC）。修复后的牙齿标本进行28天的pH循环，然后再次在偏光显微镜下分析再矿化情况。对于研究的第二部分，使用标准化黄铜模具和三种实验材料制作60个圆柱形标本（每组20个）。标本分别浸入去离子水中。浸泡7天后，每组10个标本进行显微硬度测量，并在30天结束时对其余标本重复测量。
采用单因素方差分析和事后Tukey检验进行统计分析。
CPPGIC显示出最大的再矿化，其次是GSGIC，GIC最小。三组之间的显微硬度比较无统计学意义。
葡萄籽提取物和CPPACP可用于受损牙本质的再矿化，将它们加入GIC中不会显著损害显微硬度特性。
