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高分辨率熔解 PCR 分析快速鉴定衣原体流产 1B 活疫苗株在衣原体流产菌株和田间分离株中的应用。

High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.

机构信息

Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (Anses), Maisons-Alfort, France.

出版信息

J Microbiol Methods. 2012 Sep;90(3):241-4. doi: 10.1016/j.mimet.2012.05.014. Epub 2012 May 28.

DOI:10.1016/j.mimet.2012.05.014
PMID:22652433
Abstract

We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.

摘要

我们描述了一种新型的高分辨率熔解分析方法，该方法基于先前描述的单核苷酸多态性，能够清晰地区分衣原体流产活疫苗 1B 株与田间流产衣原体菌株和田间野生型分离株。这种现代的基因分型技术价格低廉、易于使用且比 PCR-RFLP 耗时更少。

相似文献

1
High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.高分辨率熔解 PCR 分析快速鉴定衣原体流产 1B 活疫苗株在衣原体流产菌株和田间分离株中的应用。
J Microbiol Methods. 2012 Sep;90(3):241-4. doi: 10.1016/j.mimet.2012.05.014. Epub 2012 May 28.
2
Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease.基因组证据表明，活的衣原体流产疫苗株 1B 并未减毒，并且有可能引起疾病。
Vaccine. 2018 Jun 14;36(25):3593-3598. doi: 10.1016/j.vaccine.2018.05.042.
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Prevalence of chlamydiae in semen and genital tracts of bulls, rams and bucks.公牛、公羊和雄鹿精液及生殖道中衣原体的流行情况。
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The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection.1B 疫苗株沙眼衣原体导致的胎盘病变与野毒株感染无法区分。
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Evidence of Chlamydophila abortus vaccine strain 1B as a possible cause of ovine enzootic abortion.流产嗜衣原体疫苗株1B作为绵羊地方流行性流产可能病因的证据。
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J Clin Microbiol. 2005 Sep;43(9):4758-65. doi: 10.1128/JCM.43.9.4758-4765.2005.

引用本文的文献

1
Recent advances and public health implications for environmental exposure to Chlamydia abortus: from enzootic to zoonotic disease.最近在环境中接触衣原体流产的进展和公共卫生影响：从地方病到人畜共患病。
Vet Res. 2022 May 31;53(1):37. doi: 10.1186/s13567-022-01052-x.
2
Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease.基因组证据表明，活的衣原体流产疫苗株 1B 并未减毒，并且有可能引起疾病。
Vaccine. 2018 Jun 14;36(25):3593-3598. doi: 10.1016/j.vaccine.2018.05.042.
3
Diversification and Distribution of Ruminant Chlamydia abortus Clones Assessed by MLST and MLVA.
通过多位点序列分型（MLST）和多位点可变数目串联重复序列分析（MLVA）评估反刍动物流产衣原体克隆的多样性和分布
PLoS One. 2015 May 22;10(5):e0126433. doi: 10.1371/journal.pone.0126433. eCollection 2015.