Liu Qiuyue, Zhang Jin, Guo Wei, Zhao Yiqiang, Hu Xiaoxiang, Li Ning
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China.
Front Biosci (Elite Ed). 2012 Jun 1;4(7):2605-16. doi: 10.2741/e569.
Clenbuterol is a repartition agent (beta 2-adrenoceptor agonist) that can decrease fat deposition and increase skeletal muscle growth at manageable dose. To better understand the molecular mechanism of Clenbuterol's action, GeneChips and real-time PCR were used to compare the gene expression profiles of liver tissue in pigs with/without administration of Clenbuterol. Metabolism effects and the global gene expression profiles of liver tissue from Clenbuterol-treated and untreated pigs were conducted. Function enrichment tests showed that the differentially expressed genes are enriched in glycoprotein protein, plasma membrane, fatty acid and amino acid metabolic process, and cell differentiation and signal transduction groups. Pathway mining analysis revealed that physiological pathways such as MAPK, cell adhesion molecules, and the insulin signaling pathway, were remarkably regulated when Clenbuterol was administered. Gene prioritization algorithm was used to associate a number of important differentially expressed genes with lipid metabolism in response to Clenbuterol. Genes identified as differentially expressed in this study will be candidates for further investigation of the molecular mechanisms involved in Clenbuterol's effects on adipose and skeletal muscle tissue.
克伦特罗是一种重分配剂（β2-肾上腺素能受体激动剂），在可控剂量下可减少脂肪沉积并促进骨骼肌生长。为了更好地理解克伦特罗的作用分子机制，利用基因芯片和实时定量聚合酶链反应比较了给予/未给予克伦特罗的猪肝脏组织的基因表达谱。对克伦特罗处理和未处理猪的肝脏组织进行了代谢效应和整体基因表达谱分析。功能富集试验表明，差异表达基因富集于糖蛋白、质膜、脂肪酸和氨基酸代谢过程以及细胞分化和信号转导组。通路挖掘分析显示，给予克伦特罗时，丝裂原活化蛋白激酶（MAPK）、细胞黏附分子和胰岛素信号通路等生理通路受到显著调节。基因优先级算法用于将一些重要的差异表达基因与克伦特罗诱导的脂质代谢相关联。本研究中鉴定为差异表达的基因将作为进一步研究克伦特罗对脂肪和骨骼肌组织作用的分子机制的候选基因。
