Alptekin D, Izmirli M, Bayazit Y, Luleyap H U, Yilmaz M B, Soyupak B, Erkoc M A, Tansug Z
Department of Medical Biology, Faculty of Medicine, University of Cukurova, Adana, Turkey.
Genet Mol Res. 2012 May 18;11(2):1424-32. doi: 10.4238/2012.May.18.1.
The number of trinucleotide repeats [CAG (coding for polyglutamine), GGC (coding for polyglycine)] in the first exon of the androgen receptor (AR) gene and prostate-specific antigen (PSA) gene androgen response element I A/G polymorphism are both related to prostate cancer prognosis. We investigated whether these genomic changes occur in the AR and PSA genes, which are usually found in individuals with prostate cancer, of Turkish patients and to find out their distribution in the population. We used PCR and PCR-RFLP assays for AR and PSA genes, respectively, to detect molecular changes in 44 prostate cancer patients. Our findings indicate that individuals with prostate cancer tend to have around 18 CAG trinucleotide repeats. We observed significant differences between 22 controls, 33 benign prostate hyperplasia (BPH) patients and 44 adenocarcinoma patients for long CAG repeats. However, we did not find any significant differences in GGC repeats between controls, BPH and adenocarcinoma patients (P = 0.408). We also did not observe significant differences in the PSA A/G polymorphism frequency between controls, BPH and adenocarcinoma patients (P = 0.483). In conclusion, CAG and GGC repeats in the AR and PSA gene polymorphisms may be associated with prostate cancer risk and BPH in the Turkish population.
雄激素受体（AR）基因第一外显子中的三核苷酸重复序列数量[CAG（编码聚谷氨酰胺），GGC（编码聚甘氨酸）]以及前列腺特异性抗原（PSA）基因雄激素反应元件I A/G多态性均与前列腺癌预后相关。我们调查了这些基因组变化是否发生在通常在前列腺癌患者中发现的土耳其患者的AR和PSA基因中，并了解它们在人群中的分布情况。我们分别使用PCR和PCR-RFLP分析来检测44例前列腺癌患者AR和PSA基因的分子变化。我们的研究结果表明，前列腺癌患者往往有大约18个CAG三核苷酸重复序列。我们观察到22名对照、33名良性前列腺增生（BPH）患者和44名腺癌患者在长CAG重复序列方面存在显著差异。然而，我们在对照、BPH和腺癌患者之间未发现GGC重复序列有任何显著差异（P = 0.408）。我们也未观察到对照、BPH和腺癌患者在PSA A/G多态性频率方面存在显著差异（P = 0.483）。总之，AR和PSA基因多态性中的CAG和GGC重复序列可能与土耳其人群中的前列腺癌风险和BPH相关。
