Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Med Oncol. 2012 Dec;29(5):3055-62. doi: 10.1007/s12032-012-0257-1. Epub 2012 Jun 1.
Progression from chronic hepatitis C virus infection to cirrhosis then to hepatocellular carcinoma usually results in some protein changes in peripheral blood. We evaluated MAGE-4 mRNA, TGFβ1 and AFP in peripheral blood as potential biochemical markers for diagnosis and prognosis of some complications of HCV infection. MAGE-4 mRNA in blood by reverse transcription polymerase chain reaction, serum TGF-Β1 and AFP by ELISA was assayed in seventy-five individuals who were classified into five groups: group I (control) comprised fifteen apparently healthy volunteers, group II involved fifteen HCV-infected patients without cirrhosis, group III involved fifteen HCV fifteen HCV-infected patients with cirrhosis, group IV included fifteen HCV-infected patients with cirrhosis and early stage HCC, and group V included fifteen HCV cirrhotic patients and late-stage HCC. We found that the frequency of positivity of MAGE-4 among the late hepatoma group was 40 %, while in the early hepatoma group the positivity was 6.7 %. The results for TGF-Β1 revealed a significant increase in serum TGF-Β1 in groups IV and V as compared to control, II, III groups. The obtained results of AFP showed a significant positive increase in serum AFP in groups IV and V when compared to groups II and III. Detection of MAGE-4 transcripts in blood, especially with follow-up survey, may help to predict the prognosis and monitoring of the response to the therapy, and serum TGF-Β1 level in HCC patients is directly correlated with metastasis and recurrence of tumors and increases gradually with the progression of HCC.
从慢性丙型肝炎病毒感染进展为肝硬化，然后发展为肝细胞癌，通常会导致外周血中的某些蛋白质发生变化。我们评估了外周血中的 MAGE-4mRNA、TGFβ1 和 AFP，作为 HCV 感染某些并发症诊断和预后的潜在生化标志物。通过逆转录聚合酶链反应检测血液中的 MAGE-4mRNA，通过 ELISA 检测血清 TGF-Β1 和 AFP，我们对 75 名个体进行了评估，这些个体分为五组：第 I 组（对照组）包括 15 名明显健康的志愿者，第 II 组包括 15 名无肝硬化的 HCV 感染患者，第 III 组包括 15 名肝硬化的 HCV 感染患者，第 IV 组包括 15 名肝硬化和早期 HCC 的 HCV 感染患者，第 V 组包括 15 名肝硬化和晚期 HCC 的 HCV 感染患者。我们发现晚期肝癌组中 MAGE-4 的阳性率为 40%，而早期肝癌组的阳性率为 6.7%。TGF-β1 的结果显示，与对照组、第 II 组和第 III 组相比，第 IV 组和第 V 组的血清 TGF-β1 显著增加。AFP 的结果显示，与第 II 组和第 III 组相比，第 IV 组和第 V 组的血清 AFP 显著增加。在外周血中检测到 MAGE-4 转录本，特别是进行随访调查，可能有助于预测预后和监测治疗反应，并且 HCC 患者的血清 TGF-β1 水平与肿瘤的转移和复发直接相关，并且随着 HCC 的进展逐渐增加。
