Indian J Microbiol. 2011 Jun;51(2):132-7. doi: 10.1007/s12088-011-0125-2. Epub 2011 Jan 30.
A study was undertaken to investigate the presence of archaeal diversity in saltpan sediments of Goa, India by 16S rDNA-dependent molecular phylogeny. Small subunit rRNA (16S rDNA) from saltpan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. 10 unique phylotypes were obtained by PCR based RFLP of 16S rRNA genes using endonuclease Msp 1, which was most suitable to score the genetic diversity. These phylotypes spanned a wide range within the domain archaea including both crenarchaeota and euryarcheaota. None of the retrieved crenarchaeota sequences could be grouped with previously cultured crenarchaeota however; two sequences were related with haloarchaea. Most of the sequences determined were closely related to the sequences that had been previously obtained from metagenome of a variety of marine environments. The phylogenetic study of a site investigated for the first time revealed the presence of low archaeal population but showed yet unclassified species, may specially adapted to the salt pan sediment of Goa.
本研究采用基于 16S rDNA 的分子系统发育学方法，调查了印度果阿盐田沉积物中的古菌多样性。通过聚合酶链反应（PCR）扩增盐田沉积物宏基因组中的小亚基 rRNA（16S rDNA），使用针对古菌域的特异性引物。通过使用内切酶 Msp 1 的基于 PCR 的 RFLP 对 16S rRNA 基因进行分析，获得了 10 个独特的系统发育型，Msp 1 最适合评分遗传多样性。这些系统发育型在古菌域内广泛分布，包括 crenarchaeota 和 euryarcheaota。虽然没有一个回收的 crenarchaeota 序列可以与先前培养的 crenarchaeota 分组，但有两个序列与 haloarchaea 有关。大多数确定的序列与先前从各种海洋环境的宏基因组中获得的序列密切相关。对一个首次调查的地点进行的系统发育研究表明，古菌种群数量较低，但显示出尚未分类的物种，可能特别适应果阿盐田沉积物。
