Wang Qian-Fu, Li Wei, Yang Hai, Liu Yan-Li, Cao Hai-Hua, Dornmayr-Pfaffenhuemer Marion, Stan-Lotter Helga, Guo Guang-Qin
Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China.
Fachbereich Molekulare Biologie, Abt. Mikrobiologie, Billrothstrasse 11, A-5020 Salzburg, Austria.
Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):600-604. doi: 10.1099/ijs.0.64673-0.
A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5(T), was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35-40 degrees C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5(T) was most closely related to those of Halococcus species. Its pattern of antibiotic susceptibility was similar to those of other described Halococcus species. Biochemical tests revealed no sign of H(2)S production or gelatin liquefaction. The main polar lipids of strain CM5(T) were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5(T) was 61.2 mol% and it gave DNA-DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with Halococcus salifodinae DSM 8989(T), Halococcus dombrowskii DSM 14522(T) and Halococcus morrhuae ATCC 17082(T). Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5(T) should be classified within a novel species, Halococcus qingdaonensis sp. nov., with strain CM5(T) (=CGMCC 1.4243(T)=JCM 13587(T)) as the type strain.
从中国青岛附近采集的粗海盐样品中分离出一株革兰氏阴性、极端嗜盐的球状古菌菌株CM5(T)。该菌株在20%（w/v）NaCl存在下，于35 - 40℃和pH 6.0条件下生长最佳。其菌落呈红色，能够利用葡萄糖作为唯一碳源进行生长。CM5(T)的16S rRNA基因序列与盐球菌属物种的序列关系最为密切。其抗生素敏感性模式与其他已描述的盐球菌属物种相似。生化试验未显示产生H₂S或明胶液化的迹象。菌株CM5(T)的主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯和硫酸化二糖基二醚。不存在硫酸磷脂酰甘油。菌株CM5(T)的DNA G + C含量为61.2 mol%，它与盐生盐球菌DSM 8989(T)、多布罗斯基盐球菌DSM 14522(T)和莫氏盐球菌ATCC 17082(T)的DNA - DNA重结合值分别为33.7%、57.1%和29.6%。基于其形态学、化学分类学特性以及16S rRNA基因序列数据的系统发育分析，我们建议将CM5(T)归为一个新物种，即青岛盐球菌（Halococcus qingdaonensis sp. nov.），以菌株CM5(T)（=CGMCC 1.4243(T)=JCM 13587(T)）作为模式菌株。
