Chodurek Ewa, Orchel Arkadiusz, Orchel Joanna, Kurkiewicz Sławomir, Gawlik Natalia, Dzierżewicz Zofia, Stępień Krystyna
Department of Biopharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland.
ScientificWorldJournal. 2012;2012:854096. doi: 10.1100/2012/854096. Epub 2012 May 2.
The increase of a skin malignant melanoma (melanoma malignum) incidence in the world has been observed in recent years. The tumour, especially in advanced stadium with metastases, is highly resistant to conventional treatment. One of the strategies is to modulate melanogenesis using chemical compounds. In this study, the processes of differentiation and melanogenesis induced by dimethylsulfoxide (DMSO) in human melanoma cells (A-375) were investigated. Natural melanin isolated from A-375 melanoma cell line treated with 0.3% DMSO was analyzed by pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) method. The products derived from pheomelanin have not been stated in the pyrolytic profile of analyzed melanin. Within all products derived from eumelanins, 1,2-benzenediol has been predominated. It has been shown that in the melanoma cells stimulated with 0.3% and 1% DMSO, the increase of transcriptional activity of the tyrosinase gene took place. It was accompanied by the rise of tyrosinase activity and an accumulation of melanin in the cells. The better knowledge about the structure of melanins can contribute to establish the uniform criteria of malignant melanoma morbidity risk.
近年来，全球皮肤恶性黑色素瘤（恶性黑素瘤）的发病率呈上升趋势。该肿瘤，尤其是在伴有转移的晚期阶段，对传统治疗具有高度抗性。其中一种策略是使用化学化合物调节黑色素生成。在本研究中，研究了二甲基亚砜（DMSO）在人黑色素瘤细胞（A - 375）中诱导的分化和黑色素生成过程。采用热解气相色谱 - 质谱联用（Py - GC/MS）方法分析了用0.3% DMSO处理的A - 375黑色素瘤细胞系中分离出的天然黑色素。在所分析黑色素的热解图谱中未提及源自褐黑素的产物。在所有源自真黑素的产物中，邻苯二酚占主导地位。结果表明，在用0.3%和1% DMSO刺激的黑色素瘤细胞中，酪氨酸酶基因的转录活性增加。同时伴随着酪氨酸酶活性的升高以及细胞内黑色素的积累。对黑色素结构的更深入了解有助于建立恶性黑色素瘤发病风险的统一标准。
