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人 B1 细胞频率：人 B1 细胞的分离与分析。

Human b1 cell frequency: isolation and analysis of human b1 cells.

机构信息

Elmezzi Graduate School of Molecular Medicine, The Feinstein Institute for Medical Research Manhasset, NY, USA.

出版信息

Front Immunol. 2012 May 25;3:122. doi: 10.3389/fimmu.2012.00122. eCollection 2012.

DOI:10.3389/fimmu.2012.00122
PMID:22654880
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3360193/
Abstract

Controversy over the frequency of human B1 cells in normal individuals has arisen as different labs have begun to employ non-uniform techniques to study this population. The phenotypic profile and relative paucity of circulating human B1 cells place constraints on methodology to identify and isolate this population. Multiple steps must be optimized to insure accurate enumeration and optimal purification. In the course of working with human B1 cells we have developed a successful strategy that provides consistent analysis of B1 cells for frequency determination and efficient isolation of B1 cells for functional studies. Here we discuss issues attendant to identifying human B1 cells and outline a carefully optimized approach that leads to uniform and reproducible data.

摘要

关于正常人中 B1 细胞的频率存在争议，因为不同的实验室开始采用不同的技术来研究这一群体。B1 细胞的表型特征和相对稀少性限制了鉴定和分离这一群体的方法。必须优化多个步骤以确保准确计数和最佳纯化。在研究人类 B1 细胞的过程中，我们开发了一种成功的策略，该策略为频率测定提供了一致的 B1 细胞分析，为功能研究提供了高效的 B1 细胞分离。在这里，我们讨论了与鉴定人类 B1 细胞相关的问题，并概述了一种经过精心优化的方法，可得到一致和可重复的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/cf0e15de4d74/fimmu-03-00122-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/1cab12ac7349/fimmu-03-00122-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/664a79d56978/fimmu-03-00122-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/b2e35c99c664/fimmu-03-00122-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/529af5324603/fimmu-03-00122-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/784944f953eb/fimmu-03-00122-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/cf0e15de4d74/fimmu-03-00122-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/1cab12ac7349/fimmu-03-00122-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/664a79d56978/fimmu-03-00122-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/b2e35c99c664/fimmu-03-00122-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/529af5324603/fimmu-03-00122-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/784944f953eb/fimmu-03-00122-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7049/3360193/cf0e15de4d74/fimmu-03-00122-g006.jpg

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J Exp Med. 2012 Mar 12;209(3):434-6. doi: 10.1084/jem.20120403.
2
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J Exp Med. 2011 Dec 19;208(13):2566-9. doi: 10.1084/jem.20111761.
3
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Immune Netw. 2024 Mar 27;24(2):e14. doi: 10.4110/in.2024.24.e14. eCollection 2024 Apr.
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Front Immunol. 2023 Dec 15;14:1259827. doi: 10.3389/fimmu.2023.1259827. eCollection 2023.
5
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Explor Med. 2022;3(1):99-111. doi: 10.37349/emed.2022.00077. Epub 2022 Feb 25.
6
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7
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8
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9
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10
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