Takahashi T, Imai K, Sugiyama T, Sasaki T, Yachi A
Department of Internal Medicine, Sapporo Medical College, Japan.
Clin Exp Immunol. 1990 Dec;82(3):590-5. doi: 10.1111/j.1365-2249.1990.tb05495.x.
Monoclonal antibody (MoAb) KT38 raised against a human T leukaemic cell line TALL-1, reacted with another T leukaemic cell line Jurkat, but not with any other cell lines tested. The co-modulation of CD3 and KT38 antigen was observed with stimulations of either MoAb T3 or KT38 on both TALL-1 and Jurkat. Upon radioimmunoprecipitation and SDS-PAGE analysis, MoAb KT38 precipitated the heterodimer of 40-60 kD from Jurkat and TALL-1 under reducing conditions. Thus, MoAb KT38 is considered to be an anti-T idiotype (Ti) antibody to TALL-1 and Jurkat cells. MoAb KT38 was also shown to react with a minor population of peripheral blood lymphocytes (PBL) and with very few cells (0.5-2.0%) in the paracortical area of the lymph node. When PBL were stimulated in a KT38-coated culture flask for 5 days, the percentage of KT38-positive PBL was markedly increased. The CD3 antigen on these cultured PBL in the flask was modulated by the stimulation with MoAb KT38. Thus, it is suggested that a common idiotope exists on the T cell receptor of Jurkat, TALL-1 and a small percentage (1.9-6.1%) of PBL.
针对人T白血病细胞系TALL-1产生的单克隆抗体（MoAb）KT38，可与另一种T白血病细胞系Jurkat发生反应，但与所检测的任何其他细胞系均无反应。在用MoAb T3或KT38刺激TALL-1和Jurkat时，观察到CD3和KT38抗原的共同调节。经放射免疫沉淀和SDS-PAGE分析，MoAb KT38在还原条件下从Jurkat和TALL-1中沉淀出40 - 60 kD的异二聚体。因此，MoAb KT38被认为是针对TALL-1和Jurkat细胞的抗T独特型（Ti）抗体。MoAb KT38还显示与一小部分外周血淋巴细胞（PBL）以及淋巴结副皮质区中极少数细胞（0.5 - 2.0%）发生反应。当PBL在包被有KT38的培养瓶中刺激5天时，KT38阳性PBL的百分比显著增加。培养瓶中这些培养的PBL上的CD3抗原受到MoAb KT38刺激的调节。因此，提示Jurkat、TALL-1和一小部分（1.9 - 6.1%）PBL的T细胞受体上存在共同的独特型表位。
