Cohen H, Lapointe M
J Chromatogr Sci. 1979 Sep;17(9):510-3. doi: 10.1093/chromsci/17.9.510.
A procedure is described for the separation and quantification of Vitamin D3 from different feeds and premixes. The study was conducted, first using a liquid partition step as a preliminary clean-up after extraction, then chromatography on activated Silica gel 60 before final analysis on a high pressure liquid chromatograph (HPLC) using a LiChrosorb NH2 (10 mu) column and a variable wavelength UV detector set at 264 nm. Total analysis on the HPLC was achieved in fifteen minutes. The detector response curve for an authentic D3 standard was linear using peak areas with a minimum detectable amount being 5 ng. The overall percent recovery of D3 in feeds was 94.4 +/- 2.4%. The minimum detectable amount of D3 in animal feeds was found to be in the region of 2,000 I.U./kg.
本文描述了一种从不同饲料和预混料中分离和定量维生素D3的方法。研究首先采用液液分配步骤作为提取后的初步净化方法，然后在活化硅胶60上进行色谱分析，最后使用LiChrosorb NH2（10μm）柱和设定在264nm的可变波长紫外检测器在高压液相色谱仪（HPLC）上进行分析。HPLC的总分析时间为15分钟。使用峰面积时， authentic D3标准品的检测器响应曲线呈线性，最低检测量为5ng。饲料中D3的总回收率为94.4±2.4%。发现动物饲料中D3的最低检测量在2000 I.U./kg左右。
