Doheny Eye Institute, University of Southern California, Los Angeles, CA 90089, USA.
Tissue Eng Part C Methods. 2012 Dec;18(12):968-75. doi: 10.1089/ten.TEC.2012.0034. Epub 2012 Jul 19.
The study of the mechanical behavior of soft biological materials presents many challenges due to the materials' time-dependent mechanical response as well as inherent size and shape limitations. In this study, by using agar as a surrogate material for soft tissues, the effects of these limitations upon standardized macroscale dynamic compression protocols are compared to dynamic nanoindentation procedures. Both techniques are then applied to dynamically test porcine sclera tissue, showing a significant difference in recorded loss and storage modulus values between the two methodologies. Additionally, the tissues of the porcine eye wall are characterized with macrocompression in their layered in vivo arrangement. The overall constraints of standardized macroscale tests for dynamic mechanical characterization of thin and soft biological tissues are discussed.
由于软生物材料的力学行为具有时间依赖性以及固有的尺寸和形状限制，因此对其进行研究具有很大的挑战性。在本研究中，通过使用琼脂作为软组织的替代材料，将这些限制对标准化宏观动态压缩方案的影响与动态纳米压痕程序进行了比较。然后，这两种技术都被应用于动态测试猪巩膜组织，两种方法记录的损耗和储能模量值有显著差异。此外，还以猪眼壁的层状体内排列进行了宏观压缩，对其进行了组织特征描述。本文还讨论了用于动态机械特性测试的标准化宏观测试对薄而软的生物组织的整体约束。
