Gannedahl G, Fellström B, Larsson E, Roos-Engstrand E, Tufveson G
Department of Urology, University of Uppsala, Sweden.
Eur Surg Res. 1990;22(4):206-12. doi: 10.1159/000129102.
A simple technique for grafting a mouse heart heterotopically to rat neck vessels in experimental xenotransplantation is described. In this series, graft survival was tested with the following modes of immunomodulation: ciclosporin (Cy), athymic and nude rats, splenectomy, total lymphoid irradiation (TLI), 15-deoxyspergualin in two different doses and various combinations of these. The mean graft survival time, with Cy or athymic and nude rats as recipients, was 3 days as also seen in animals without treatment. With either irradiation, splenectomy or 15-deoxyspergualin, 10 mg/kg daily, the graft survival time was prolonged to 7.8, 4.7 and 6.8 days, respectively. In combination, TLI + splenectomy gave a graft survival of 9.7 days and Cy + splenectomy 7.2 days. With 15-deoxyspergualin at 10 mg/kg/day plus splenectomy, done 1 week prior to transplantation, graft survival was extended to 17.8 days.
本文描述了一种在实验性异种移植中将小鼠心脏异位移植到大鼠颈部血管的简单技术。在本系列实验中，通过以下免疫调节方式测试移植物的存活情况：环孢素（Cy）、无胸腺大鼠和裸大鼠、脾切除术、全身淋巴照射（TLI）、两种不同剂量的15-去氧精胍菌素以及这些方法的各种组合。以Cy或无胸腺大鼠和裸大鼠作为受体时，平均移植物存活时间为3天，未治疗的动物也是如此。采用照射、脾切除术或每日10mg/kg的15-去氧精胍菌素治疗时，移植物存活时间分别延长至7.8天、4.7天和6.8天。联合使用时，TLI +脾切除术使移植物存活9.7天，Cy +脾切除术使移植物存活7.2天。在移植前1周进行每日10mg/kg的15-去氧精胍菌素加脾切除术，移植物存活时间延长至17.8天。
