Siemieniak D R, Slightom J L
Molecular Biology Unit Upjohn Company, Kalamazoo, MI 49007.
Gene. 1990 Nov 30;96(1):121-4. doi: 10.1016/0378-1119(90)90350-z.
Primer-directed sequencing, of double-stranded large recombinant DNA molecules, has not been accepted because of the delay and expenses involved in the synthesis of oligodeoxyribonucleotide primers. A potential solution to this problem was proposed by Studier [Proc. Natl. Acad. Sci. USA 86 (1989) 6917-6921] for using a library of short oligomer primers. Szybalski [Gene 90 (1990) 177-178] has made a complementary proposal using ligated hexamers to reduce the number of oligomers needed. We have used a set of rules for a computer-aided selection of a library consisting of 3342 specific nonamers. The effectiveness of this library of nonamers to sequence specific genes was studied using human sequences available in GenBank.
由于合成寡脱氧核糖核苷酸引物存在延迟和费用问题，双链大重组DNA分子的引物定向测序尚未被广泛接受。Studier [美国国家科学院院刊86 (1989) 6917 - 6921] 提出了一个潜在的解决方案，即使用短寡聚物引物文库。Szybalski [基因90 (1990) 177 - 178] 提出了一个互补方案，即使用连接的六聚体来减少所需寡聚物的数量。我们使用了一组规则，通过计算机辅助选择了一个由3342个特定九聚体组成的文库。利用GenBank中可用的人类序列研究了这个九聚体文库对特定基因进行测序的有效性。
