Program of Biomedical Engineering, School of Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.
Biomacromolecules. 2012 Jul 9;13(7):2202-10. doi: 10.1021/bm3006227. Epub 2012 Jun 13.
Antibiotic delivery is important to treat bacterial infections, one of the most challenging health problems globally. This study explored the application of nucleic acids as an antibiotic-binding effector for antibiotic loading and release. The data showed that the partition coefficient of tetracycline increased proportionally to the oligonucleotide concentration ranging from 0 to 1 mM. Resultantly, the incorporation of the oligonucleotides led to enhanced tetracycline loading in the hydrogels. In addition to the enhanced drug loading, the oligonucleotides could slow the release of tetracycline from the hydrogels. Experiments were further carried out to examine the capability of oligonucleotide-functionalized hydrogels in the inhibition of bacterial growth. The results showed that the oligonucleotide-functionalized hydrogels had higher antibacterial efficiency. Moreover, after tetracycline release, the oligonucleotide-functionalized hydrogels could be refilled with fresh tetracycline to reproduce the capability of inhibiting bacterial growth. Therefore, nucleic acid oligonucleotides are a promising antibiotic-binding effector for hydrogel functionalization in antibiotic delivery.
抗生素的递送对于治疗细菌感染至关重要，而细菌感染是全球面临的最具挑战性的健康问题之一。本研究探索了核酸作为抗生素结合效应物在抗生素负载和释放方面的应用。研究数据表明，四环素的分配系数与寡核苷酸浓度成正比，范围从 0 到 1mM。结果表明，寡核苷酸的掺入导致水凝胶中四环素的负载量增加。除了增强药物负载外，寡核苷酸还可以减缓四环素从水凝胶中的释放。进一步进行了实验以研究寡核苷酸功能化水凝胶在抑制细菌生长方面的能力。结果表明，寡核苷酸功能化水凝胶具有更高的抗菌效率。此外，在释放四环素后，寡核苷酸功能化水凝胶可以用新鲜的四环素重新填充，以重现抑制细菌生长的能力。因此，核酸寡核苷酸是水凝胶功能化用于抗生素递送的有前途的抗生素结合效应物。
