Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to Piso, Pabellón 2, 1428 Buenos Aires, Argentina.
Fungal Biol. 2012 Jun;116(6):729-35. doi: 10.1016/j.funbio.2012.04.007. Epub 2012 Apr 22.
Establishment of arbuscular mycorrhizal (AM) germplasm collections is complex because of the obligate biotrophic nature of AM fungi. Only a few AM species are routinely maintained in monoxenic culture with Ri T-DNA transformed roots as host. Incorporation of new AM species into this culture system is important for molecular, physiological, and taxonomical studies. Here we report for the first time the successful monoxenic culture of Gigaspora decipiens (JA2 strain) with transformed carrot (Daucus carota) roots. In vitro cultures were established from field-collected spores; sub-culture of newly in vitro formed spores was established over five successive generations for a period of 6 y. Although initial culture of field-collected spores was difficult successive sub-cultures appeared to be adapted to the in vitro growing conditions. The JA2 strain of G. decipiens completed its life cycle while maintaining its morphological characteristics, stability, and propagule viability under the monoxenic conditions over several generations. This stable and homogeneous monoxenic material obtained for G. decipiens is part of the Banco de Glomeromycota In Vitro (BGIV, http://www.bgiv.com.ar), and could facilitate morphological, physiological, and molecular analysis of this AM species.
丛枝菌根（AM）种质资源的建立较为复杂，因为 AM 真菌具有专性的生物营养特性。只有少数 AM 物种能够在以 Ri T-DNA 转化根为宿主的单养培养中常规维持。将新的 AM 物种纳入该培养系统对于分子、生理和分类学研究非常重要。在这里，我们首次成功地用转化胡萝卜（Daucus carota）根对巨型内养孢囊菌（JA2 菌株）进行了单养培养。体外培养是从田间收集的孢子中建立的；新体外形成的孢子的次培养在 6 年的时间里连续进行了五代。尽管最初培养田间收集的孢子很困难，但随后的次培养似乎适应了体外生长条件。JA2 菌株的巨型内养孢囊菌在单养条件下完成了其生命周期，同时保持了其形态特征、稳定性和繁殖体活力，经过几代。这种巨型内养孢囊菌获得的稳定和均匀的单养材料是 Banco de Glomeromycota In Vitro（BGIV，http://www.bgiv.com.ar）的一部分，可促进该 AM 物种的形态、生理和分子分析。
