增强型多重聚合酶链反应检测耐甲氧西林金黄色葡萄球菌中 I 至 V 型葡萄球菌盒式染色体 mec 类型。
Enhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.
机构信息
Centre for Antimicrobial Resistance, Alberta Health Services/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada.
出版信息
Mol Cell Probes. 2012 Oct;26(5):218-21. doi: 10.1016/j.mcp.2012.04.002. Epub 2012 Apr 21.
PMID:22659351
Abstract
We previously published a simple multiplex PCR assay for quick-screening of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V, which has been extensively used worldwide. An updated assay is described here and the changes serve to make this rapid assay more accurate and reliable in facilitating identification of most common and major SCCmec types.
摘要
我们之前发表了一种简单的多重 PCR 检测方法，用于快速筛查 I-V 型葡萄球菌盒式染色体 mec（SCCmec），该方法已在全球范围内广泛使用。本文介绍了一种更新的检测方法，这些变化旨在使这种快速检测方法在鉴定最常见和主要的 SCCmec 类型时更加准确可靠。
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