泰国和越南耐甲氧西林金黄色葡萄球菌分离株中葡萄球菌盒式染色体mec型的揭示
Revelation of staphylococcal cassette chromosome mec types in methicillin-resistant Staphylococcus aureus isolates from Thailand and Vietnam.
作者信息
Lawung R, Chuong L V, Cherdtrakulkiat R, Srisarin A, Prachayasittikul V
机构信息
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Center of Medical Laboratory Services, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand; Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
出版信息
J Microbiol Methods. 2014 Dec;107:8-12. doi: 10.1016/j.mimet.2014.08.024. Epub 2014 Sep 6.
Methicillin resistant Staphylococcus aureus (MRSA) is highly prevalent, and its typing plays a crucial role in epidemiology and evolution in both health and community settings. Multiplex PCR and staphylococcal cassette chromosome mec (SCCmec) typing based on mec complexes and cassette chromosome recombinase (ccr) allotypes have been developed for MRSA identification. The first of these procedures can identify 4 mec classes (A, B, C1, and E) and 2 ccr allotypes (B2 and B4) in one tube, and the second can identify mecA, mec class C2, and 3 allotypes (A1, A3, and C). Our method offers a novel means to further differentiate between the main SCCmec types I through XI and is both highly sensitive (detectable up to 0.3ηg DNA) and specific (100%). Several SCCmec types (I, III, IV, V and a non-typeable group) were found in 66 MRSA isolates obtained from Ho Chi Minh City, Vietnam and Nakhon Pathom, Thailand. SCCmec type III was highly predominant in both regions. The designed assay is rapid, convenient, flexible, and reliable. Therefore, this assay is suitable for the high-throughput screening of the main SCCmec types of MRSA isolates.
耐甲氧西林金黄色葡萄球菌（MRSA）非常普遍，其分型在医疗和社区环境中的流行病学及进化方面起着关键作用。基于mec复合体和盒式染色体重组酶（ccr）同种异型的多重聚合酶链反应（PCR）和葡萄球菌盒式染色体mec（SCCmec）分型已被开发用于MRSA鉴定。第一种方法可在一管中鉴定4种mec类别（A、B、C1和E）和2种ccr同种异型（B2和B4），第二种方法可鉴定mecA、mec C2类别和3种同种异型（A1、A3和C）。我们的方法提供了一种新手段，可进一步区分主要的I至XI型SCCmec，且具有高灵敏度（可检测低至0.3ηg DNA）和特异性（100%）。在从越南胡志明市和泰国佛统府获得的66株MRSA分离株中发现了几种SCCmec类型（I、III、IV、V和一个不可分型组）。SCCmec III型在两个地区都占主导地位。所设计的检测方法快速、便捷、灵活且可靠。因此，该检测方法适用于MRSA分离株主要SCCmec类型的高通量筛选。
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