Medical College, Hubei University of Arts and Science, Xiangyang, 441053, China.
Xiangyang Central Hospital, The Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, China.
Braz J Microbiol. 2024 Jun;55(2):1305-1315. doi: 10.1007/s42770-024-01270-9. Epub 2024 Feb 16.
Staphylococcus aureus is a common clinical pathogen that causes various human infections. The aim of this study was to investigate the antibiotic susceptibility pattern, molecular epidemiological characteristics, and biofilm formation ability of S. aureus isolates from clinical specimens in Xiangyang and to analyze the correlation among them. A total of 111 non-duplicate S. aureus isolates were collected from the Affiliated Hospital of Hubei University of Arts and Science. All isolates were tested for antibacterial susceptibility. Methicillin-resistant S. aureus (MRSA) was identified by the mecA gene PCR amplification. All isolates were analyzed to determine their biofilm-forming ability using the microplate method. The biofilm-related gene was determined using PCR. SCCmec, MLST, and spa types of MRSA strains were performed to ascertain the molecular characteristics. Among the 111 S. aureus isolates, 45 (40.5%) and 66 (59.5%) were MRSA and MSSA, respectively. The resistance of MRSA strains to the tested antibiotics was significantly stronger than that of MSSA strains. All isolates were able to produce biofilm with levels ranging from strong (28.9%, 18.2%), moderate (62.2%, 62.1%), to weak (8.9%, 19.7%). Strong biofilm formation was observed in MRSA strains than in MSSA strains, based on percentages. There were dynamic changes in molecular epidemic characteristics of MRSA isolates in Xiangyang. SCCmecIVa-ST22-t309, SCCmecIVa-ST59-t437, and SCCmecIVa-ST5-t2460 were currently the main epidemic clones in this region. SCCmecIVa-ST5-t2460 and SCCmecIVa/III-ST22-t309 have stronger antibiotic resistance than SCCmecIVa-ST59-t437 strains, with resistance to 6 ~ 8 detected non-β-lactam antibiotics. The molecular epidemic and resistance attributes of S. aureus should be timely monitored, and effective measures should be adopted to control the clinical infection and spread of the bacteria.
金黄色葡萄球菌是一种常见的临床病原体，可引起各种人类感染。本研究旨在调查襄阳市临床标本中金黄色葡萄球菌分离株的抗生素敏感性模式、分子流行病学特征和生物膜形成能力，并分析它们之间的相关性。从湖北文理学院附属医院共采集了 111 份非重复的金黄色葡萄球菌分离株。所有分离株均进行了抗菌药物敏感性试验。采用 mecA 基因 PCR 扩增法鉴定耐甲氧西林金黄色葡萄球菌（MRSA）。采用微孔板法测定所有分离株的生物膜形成能力。采用 PCR 法检测生物膜相关基因。对 MRSA 菌株的 SCCmec、MLST 和 spa 型进行分析，以确定其分子特征。在 111 株金黄色葡萄球菌中，MRSA 和 MSSA 分别为 45 株（40.5%）和 66 株（59.5%）。MRSA 菌株对所检测抗生素的耐药性明显强于 MSSA 菌株。所有分离株均能产生生物膜，水平从强（28.9%，18.2%）、中（62.2%，62.1%）到弱（8.9%，19.7%）不等。MRSA 菌株的强生物膜形成比例高于 MSSA 菌株。襄阳市 MRSA 分离株的分子流行特征存在动态变化。SCCmecIVa-ST22-t309、SCCmecIVa-ST59-t437 和 SCCmecIVa-ST5-t2460 是该地区目前的主要流行克隆。SCCmecIVa-ST5-t2460 和 SCCmecIVa/III-ST22-t309 比 SCCmecIVa-ST59-t437 菌株具有更强的抗生素耐药性，对 6~8 种非β-内酰胺类抗生素耐药。应及时监测金黄色葡萄球菌的分子流行和耐药属性，并采取有效措施控制细菌的临床感染和传播。
