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从寻常型天疱疮患者中分离出的循环抗桥粒芯糖蛋白 3 自身抗体的致病活性。

Pathogenic activity of circulating anti-desmoglein-3 autoantibodies isolated from pemphigus vulgaris patients.

机构信息

Department of Dermatology, Medical University of Lodz, Poland.

出版信息

Arch Med Sci. 2012 May 9;8(2):347-56. doi: 10.5114/aoms.2012.28564.

DOI:10.5114/aoms.2012.28564
PMID:22662010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3361049/
Abstract

INTRODUCTION

There are scarce data on immunochemical properties of pemphigus antibodies detected in clinical remission in pemphigus vulgaris (PV) patients. The aim of the study was to compare biological activity of anti-Dsg3 autoantibodies purified from the sera of PV patients in active stage and in clinical remission.

MATERIAL AND METHODS

The effect of purified antibodies on expression of procaspase-3, Bax, Bcl-2, uPAR, IL-1β, IL-6, and TNF-α mRNAs in the HaCaT keratinocytes was evaluated by Western blot and RT-PCR method.

RESULTS

Incubation of HaCaT cells with anti-Dsg-3 autoantibodies caused their binding to cell membranes surfaces. Anti-Dsg3 autoantibodies isolated from the patients in active stage and clinical remission showed proapoptotic effect, caused enhanced expression of analyzed proinflammatory cytokines' mRNAs and uPAR mRNA.

CONCLUSIONS

Our data revealed similar pathogenic activity of anti Dsg-3 autoantibodies isolated from active and clinical remission PV patients.

摘要

简介

关于天疱疮寻常型（PV）患者临床缓解期检测到的天疱疮抗体的免疫化学特性，数据稀缺。本研究的目的是比较处于活动期和临床缓解期的 PV 患者血清中纯化的抗 Dsg3 自身抗体的生物学活性。

材料与方法

采用 Western blot 和 RT-PCR 法检测纯化抗体对 HaCaT 角质形成细胞中 procaspase-3、Bax、Bcl-2、uPAR、IL-1β、IL-6 和 TNF-α mRNA 表达的影响。

结果

抗 Dsg-3 自身抗体与 HaCaT 细胞结合。从活动期和临床缓解期患者中分离出的抗 Dsg3 自身抗体表现出促凋亡作用，导致分析的促炎细胞因子和 uPAR mRNA 的表达增强。

结论

我们的数据显示，从活动期和临床缓解期 PV 患者中分离出的抗 Dsg-3 自身抗体具有相似的致病活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/44f9bf59c232/AMS-8-18563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/2bb4a971ad97/AMS-8-18563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/2a333eaf8dd2/AMS-8-18563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/5971567797a4/AMS-8-18563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/44f9bf59c232/AMS-8-18563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/2bb4a971ad97/AMS-8-18563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/2a333eaf8dd2/AMS-8-18563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/5971567797a4/AMS-8-18563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4491/3361049/44f9bf59c232/AMS-8-18563-g004.jpg

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