Chemical and Materials Engineering Department, University of Alberta, Edmonton, AB, Canada.
Biointerphases. 2012 Dec;7(1-4):40. doi: 10.1007/s13758-012-0040-z. Epub 2012 Jun 5.
Protein-surface interactions are crucial to the overall biocompatability of biomaterials, and are thought to be the impetus towards the adverse host responses such as blood coagulation and complement activation. Only a few studies hint at the ultra-low fouling potential of zwitterionic poly(carboxybetaine methacrylate) (PCBMA) grafted surfaces and, of those, very few systematically investigate their non-fouling behavior. In this work, single protein adsorption studies as well as protein adsorption from complex solutions (i.e. human plasma) were used to evaluate the non-fouling potential of PCBMA grafted silica wafers prepared by nitroxide-mediated free radical polymerization. PCBMAs used for surface grafting varied in charge separating spacer groups that influence the overall surface charges, and chain end-groups that influence the overall hydrophilicity, thereby, allows a better understanding of these effects towards the protein adsorption for these materials. In situ ellipsometry was used to quantify the adsorbed layer thickness and adsorption kinetics for the adsorption of four proteins from single protein buffer solutions, viz, lysozyme, α-lactalbumin, human serum albumin and fibrinogen. Total amount of protein adsorbed on surfaces differed as a function of surface properties and protein characteristics. Finally, immunoblots results showed that human plasma protein adsorption to these surfaces resulted, primarily, in the adsorption of human serum albumin, with total protein adsorbed amounts being the lowest for PCBMA-3 (TEMPO). It was apparent that surface charge and chain hydrophilicity directly influenced protein adsorption behavior of PCBMA systems and are promising materials for biomedical applications.
蛋白质-表面相互作用对生物材料的整体生物相容性至关重要，并且被认为是导致血液凝固和补体激活等不良宿主反应的动力。只有少数研究提示了两性离子聚（羧基甜菜碱甲基丙烯酸酯）（PCBMA）接枝表面的超低污染潜力，而在这些研究中，很少有系统地研究它们的非污染行为。在这项工作中，使用单蛋白吸附研究以及来自复杂溶液（即人血浆）的蛋白吸附来评估通过氮氧化物介导的自由基聚合制备的 PCBMA 接枝硅片的非污染潜力。用于表面接枝的 PCBMAs 在电荷分离间隔基上有所不同，这些间隔基影响整体表面电荷，而链端基影响整体亲水性，从而可以更好地理解这些因素对这些材料的蛋白质吸附的影响。原位椭圆光度法用于定量从单蛋白缓冲溶液中吸附四种蛋白质（即溶菌酶、α-乳白蛋白、人血清白蛋白和纤维蛋白原）的吸附层厚度和吸附动力学。表面性质和蛋白质特性的不同导致了吸附在表面上的蛋白质总量的不同。最后，免疫印迹结果表明，这些表面与人血浆蛋白的吸附主要导致人血清白蛋白的吸附，而 PCBMA-3（TEMPO）的总蛋白吸附量最低。显然，表面电荷和链亲水性直接影响 PCBMA 系统的蛋白质吸附行为，是生物医学应用的有前途的材料。
