Department of Polymer Engineering, University of Akron, Akron, Ohio 44325, United States.
Langmuir. 2012 Jun 26;28(25):9700-6. doi: 10.1021/la301561j. Epub 2012 Jun 7.
In this work, a new antifouling silica hydrogel was developed for potential biomedical applications. A zwitterionic polymer, poly(carboxybetaine methacrylate) (pCBMA), was produced via atom-transfer radical polymerization and was appended to the hydrogel network in a two-step acid-base-catalyzed sol-gel process. The pCBMA silica aerogels were obtained by drying the hydrogels under supercritical conditions using CO(2). To understand the effect of pCBMA on the gel structure, pCBMA silica aerogels with different pCBMA contents were characterized using scanning electron microscopy (SEM), nuclear magnetic resonance (NMR) spectroscopy, and the surface area from Brauner-Emmet-Teller (BET) measurements. The antifouling property of pCBMA silica hydrogel to resist protein (fibrinogen) adsorption was measured using enzyme-linked immunosorbent assay (ELISA). SEM images revealed that the particle size and porosity of the silica network decreased at low pCBMA content and increased at above 33 wt % of the polymer. The presence of pCBMA increased the surface area of the material by 91% at a polymer content of 25 wt %. NMR results confirmed that pCBMA was incorporated completely into the silica structure at a polymer content below 20 wt %. A protein adsorption test revealed a reduction in fibrinogen adsorption by 83% at 25 wt % pCBMA content in the hydrogel compared to the fibrinogen adsorption in the unmodified silica hydrogel.
在这项工作中，开发了一种新的用于潜在生物医学应用的抗污硅胶水凝胶。通过原子转移自由基聚合生产两性离子聚合物聚（羧酸甜甲基丙烯酰胺）（pCBMA），并通过两步酸碱催化溶胶-凝胶过程将其接枝到水凝胶网络中。通过使用 CO2 在超临界条件下干燥水凝胶，得到 pCBMA 硅胶气凝胶。为了了解 pCBMA 对凝胶结构的影响，使用扫描电子显微镜（SEM）、核磁共振（NMR）光谱和比表面积的 Brunauer-Emmett-Teller（BET）测量来表征具有不同 pCBMA 含量的 pCBMA 硅胶气凝胶。使用酶联免疫吸附测定（ELISA）测量 pCBMA 硅胶水凝胶抵抗蛋白质（纤维蛋白原）吸附的抗污性能。SEM 图像显示，在低 pCBMA 含量下，硅胶网络的粒径和孔隙率减小，而在高于 33wt%的聚合物含量下增加。在聚合物含量为 25wt%时，pCBMA 的存在将材料的表面积增加了 91%。NMR 结果证实，在聚合物含量低于 20wt%时，pCBMA 完全掺入硅胶结构中。蛋白质吸附测试表明，与未改性的硅胶水凝胶相比，水凝胶中 pCBMA 含量为 25wt%时，纤维蛋白原吸附减少了 83%。
