Uzé M, Potrykus I, Sautter C
ETH Zürich, Institute of Plant Sciences, Universitätstr. 2, CH-8092 Zürich, Switzerland, CH.
Theor Appl Genet. 1999 Aug;99(3-4):487-95. doi: 10.1007/s001220051261.
Two non-linked marker genes (gus and bar) were co-introduced by microprojectile bombardment into wheat cells. Four different DNA structures were compared with respect to ability to integrate into the wheat genome: circular or linear (l) DNA as a single- or double-stranded plasmid (ss and ds, respectively). In eight independent experiments, linearized DNA integrated in the ds or ss form with a high efficiency of up to 14% for l-ssDNA. Molecular analyses by Southern blotting showed that all DNA forms gave a similar complicated integration pattern of the bar gene.
通过微粒轰击将两个不连锁的标记基因（gus和bar）共同导入小麦细胞。比较了四种不同的DNA结构整合到小麦基因组中的能力：环状或线性（l）DNA，分别为单链或双链质粒（分别为ss和ds）。在八项独立实验中，线性化DNA以ds或ss形式整合，l-ssDNA的整合效率高达14%。Southern印迹法的分子分析表明，所有DNA形式的bar基因都呈现出相似的复杂整合模式。
