Djian-Caporalino C, Pijarowski L, Januel A, Lefebvre V, Daubèze A, Palloix A, Dalmasso A, Abad P
Laboratoire de Biologie des Invertébrés, Institut National de la Recherche Agronomique,06600 Antibes Cedex, B.P. 2078, France, FR.
Theor Appl Genet. 1999 Aug;99(3-4):496-502. doi: 10.1007/s001220051262.
Capsicum annuum L. has resistance to root-knot nematodes (RKN) (Meloidogyne spp.), severe polyphagous pests that occur world-wide. Several single dominant genes confer this resistance. Some are highly specific, whereas others are effective against a wide range of species. The spectrum of resistance to eight clonal RKN populations of the major Meloidogyne species, M. arenaria (2 populations), M. incognita (2 populations), M. javanica (1 population), and M. hapla (3 populations) was studied using eight lines of Capsicum annuum. Host susceptibility was determined by counting the egg masses (EM) on the roots. Plants were classified into resistant (R; EM ≤ 5) or susceptible (H; EM >5) classes. The french cultivar Doux Long des Landes was susceptible to all nematodes tested. The other seven pepper lines were highly resistant to M. arenaria, M. javanica and one population of M. hapla. Variability in resistance was observed for the other two populations of M. hapla. Only lines PM687, PM217, Criollo de Morelos 334 and Yolo NR were resistant to M. incognita. To investigate the genetic basis of resistance in the highly resistant line PM687, the resistance of two progenies was tested with the two populations of M. incognita: 118 doubled-haploid (DH) lines obtained by androgenesis from F(1) hybrids of the cross between PM687 and the susceptible cultivar Yolo Wonder, and 163 F(2) progenies. For both nematodes populations, the segregation patterns 69 R / 49 S for DH lines and 163 R / 45 S for F(2) progenies were obtained at 22°C and at high temperatures (32°C and 42°C). The presence of a single dominant gene that totally prevented multiplication of M. incognita was thus confirmed and its stability at high temperature was demonstrated. This study confirmed the value of C. annuum as a source of complete spectrum resistance to the major RKN.
辣椒（Capsicum annuum L.）对根结线虫（RKN，Meloidogyne spp.）具有抗性，根结线虫是一种在全球范围内发生的严重多食性害虫。有几个单一显性基因赋予这种抗性。有些抗性具有高度特异性，而其他抗性则对多种线虫有效。利用8个辣椒品系，研究了对主要根结线虫种类（南方根结线虫（Meloidogyne arenaria，2个群体）、爪哇根结线虫（M. javanica，1个群体）、花生根结线虫（M. hapla，3个群体））的8个克隆RKN群体的抗性谱。通过统计根上的卵块（EM）来确定宿主的易感性。将植株分为抗性（R；EM≤5）或易感（H；EM>5）类别。法国品种Doux Long des Landes对所有测试的线虫均易感。其他7个辣椒品系对南方根结线虫、爪哇根结线虫和一个花生根结线虫群体具有高度抗性。观察到另外两个花生根结线虫群体的抗性存在差异。只有品系PM687, PM217, Criollo de Morelos 334和Yolo NR对爪哇根结线虫具有抗性。为了研究高抗品系PM687抗性的遗传基础，用两个爪哇根结线虫群体对两个后代的抗性进行了测试：通过雄核发育从PM687与易感品种Yolo Wonder杂交的F(1)杂种获得的118个双单倍体（DH）品系，以及163个F(2)后代。对于这两个线虫群体，在22°C和高温（32°C和42°C）下，DH品系的分离模式为69R/49S，F(2)后代的分离模式为163R/45S。因此，证实了存在一个完全阻止爪哇根结线虫繁殖的单一显性基因，并证明了其在高温下的稳定性。本研究证实了辣椒作为主要根结线虫全谱抗性来源的价值。
