Liu Clarke J H, Chèvre A M, Landgren M, Glimelius K
Department of Plant Biology, The Swedish University of Agricultural Sciences, Uppsala Genetic Centere, 7080, S-750 07, Uppsala, Sweden, SE.
Theor Appl Genet. 1999 Aug;99(3-4):605-10. doi: 10.1007/s001220051275.
Cytogenetic studies were performed on four male-sterile progenies derived from four different cybrids produced between Brassica napus and B. tournefortii using the donor-recipient protoplast fusion method. The objective of these studies was to characterize the nuclear constitution of the plants. Mitotic investigation revealed that three of the four male-sterile lines had 38 chromosomes, which is equal to that of B. napus. The fourth line, C6, had variable chromosome numbers, ranging from 39 to 42 in different plants. The meiotic behavior in each progeny varied distinctly. Of the plants having 38 chromosomes, fairly high chromosome pairing, on average 18.08 bivalents per cell, was detected at metaphase-I. However, univalents with an average of 1.39 per cell, and very low frequencies of trivalents and/or tetravalents, were also observed in the lines. These results revealed that male-sterile cybrid lines were obtained with 38 chromosomes and a relatively high level of chromosome-pairing ability, indicating their potential for establishing a stable male-sterile rapeseed line.
利用供体-受体原生质体融合方法，对甘蓝型油菜（Brassica napus）和图尔恩福氏油菜（B. tournefortii）之间产生的四个不同胞质杂种衍生的四个雄性不育后代进行了细胞遗传学研究。这些研究的目的是表征这些植物的核组成。有丝分裂研究表明，四个雄性不育系中的三个有38条染色体，与甘蓝型油菜的染色体数相同。第四个品系C6在不同植株中的染色体数不同，从39条到42条不等。每个后代的减数分裂行为明显不同。在具有38条染色体的植株中，在中期I检测到相当高的染色体配对，平均每个细胞有18.08个二价体。然而，在这些品系中也观察到平均每个细胞有1.39个单价体，以及三价体和/或四价体的频率非常低。这些结果表明，获得了具有38条染色体和相对较高染色体配对能力的雄性不育胞质杂种系，表明它们具有建立稳定雄性不育油菜品系的潜力。
