D'Orsogna Lloyd J, van der Meer-Prins Ellen M W, Zoet Yvonne M, Roelen Dave L, Doxiadis Ilias I N, Claas Frans H J
Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands.
Methods Mol Biol. 2012;882:339-49. doi: 10.1007/978-1-61779-842-9_19.
The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.
使用荧光染料标记的四聚体复合物直接测量病毒特异性淋巴细胞的能力已被证明是移植领域的一项重大进展。病毒肽/HLA四聚体复合物可利用单细胞分选设备快速生成病毒特异性克隆，从而能够从具有已知特异性的单个TCR确定同种异体反应性。当与称为单HLA抗原转染的K562细胞（SALs）的新型靶“检测”细胞结合使用时，人类同种异体反应首次能够得到特异性和可靠的检测。在此，我们描述了一种使用表达单HLA的细胞系作为同种异体靶标从病毒特异性记忆T细胞中检测“异源免疫”的方法。
